dc.contributor.author
Nikolay, Rainer
dc.contributor.author
Schloemer, Renate
dc.contributor.author
Mueller, Silke
dc.contributor.author
Deuerling, Elke
dc.date.accessioned
2018-06-08T03:25:18Z
dc.date.available
2015-03-26T11:05:15.189Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15148
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19336
dc.description.abstract
Background Ribosomes and functional complexes of them have been analyzed at
the atomic level. Far less is known about the dynamic assembly and degradation
events that define the half-life of ribosomes and guarantee their quality
control. Results We developed a system that allows visualization of intact
ribosomal subunits and assembly intermediates (i.e. assembly landscapes) by
convenient fluorescence-based analysis. To this end, we labeled the early
assembly ribosomal proteins L1 and S15 with the fluorescent proteins mAzami
green and mCherry, respectively, using chromosomal gene insertion. The
reporter strain harbors fluorescently labeled ribosomal subunits that operate
wild type-like, as shown by biochemical and growth assays. Using genetic and
chemical perturbations by depleting genes encoding the ribosomal proteins L3
and S17, respectively, or using ribosome-targeting antibiotics, we provoked
ribosomal subunit assembly defects. These defects were readily identified by
fluorometric analysis after sucrose density centrifugation in unprecedented
resolution. Conclusion This strategy is useful to monitor and characterize
subunit specific assembly defects caused by ribosome-targeting drugs that are
currently used and to characterize new molecules that affect ribosome assembly
and thereby constitute new classes of antibacterial agents.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Fluorescence-based monitoring of ribosome assembly landscapes
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
BMC Molecular Biology. - 16 (2015), Artikel Nr. 3
dcterms.bibliographicCitation.doi
10.1186/s12867-015-0031-y
dcterms.bibliographicCitation.url
http://www.biomedcentral.com/1471-2199/16/3
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022102
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004707
dcterms.accessRights.openaire
open access