dc.contributor.author
Koller, Barbara
dc.contributor.author
Schramm, Christin
dc.contributor.author
Siebert, Susann
dc.contributor.author
Triebel, János
dc.contributor.author
Deland, Eric
dc.contributor.author
Pfefferkorn, Anna M.
dc.contributor.author
Rickerts, Volker
dc.contributor.author
Thewes, Sascha
dc.date.accessioned
2018-06-08T03:22:49Z
dc.date.available
2016-11-21T08:16:53.537Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15057
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19245
dc.description.abstract
The social amoeba Dictyostelium discoideum is a well-established model
organism to study the interaction between bacteria and phagocytes. In
contrast, research using D. discoideum as a host model for fungi is rare. We
describe a comprehensive study, which uses D. discoideum as a host model
system to investigate the interaction with apathogenic (Saccharomyces
cerevisiae) and pathogenic (Candida sp.) yeast. We show that Dictyostelium can
be co-cultivated with yeasts on solid media, offering a convenient test to
study the interaction between fungi and phagocytes. We demonstrate that a
number of D. discoideum mutants increase (atg1−, kil1−, kil2−) or decrease
(atg6−) the ability of the amoebae to predate yeast cells. On the yeast side,
growth characteristics, reduced phagocytosis rate, as well as known virulence
factors of C. albicans (EFG1, CPH1, HGC1, ICL1) contribute to the resistance
of yeast cells against predation by the amoebae. Investigating haploid C.
albicans strains, we suggest using the amoebae plate test for screening
purposes after random mutagenesis. Finally, we discuss the potential of our
adapted amoebae plate test to use D. discoideum for risk assessment of yeast
strains.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
host-pathogeninteraction
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Dictyostelium discoideum as a Novel Host System to Study the Interaction
between Phagocytes and Yeasts
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Frontiers in Microbiology 7:1665
dcterms.bibliographicCitation.doi
10.3389/fmicb.2016.01665
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3389/fmicb.2016.01665
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.affiliation.other
Institut für Biologie
refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
refubium.mycore.fudocsId
FUDOCS_document_000000025727
refubium.note.author
Gefördert durch die DFG und den Open-Access-Publikationsfonds der Freien
Universität Berlin.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007368
dcterms.accessRights.openaire
open access