dc.contributor.author
Conrad, Thomas
dc.contributor.author
Albrecht, Anne-Susann
dc.contributor.author
Costa, Veronica Rodrigues de Melo
dc.contributor.author
Sauer, Sascha
dc.contributor.author
Meierhofer, David
dc.contributor.author
Orom, Ulf Andersson
dc.date.accessioned
2018-06-08T03:14:14Z
dc.date.available
2016-05-17T09:07:11.260Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14755
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18945
dc.description.abstract
Novel RNA-guided cellular functions are paralleled by an increasing number of
RNA-binding proteins (RBPs). Here we present ‘serial RNA interactome capture’
(serIC), a multiple purification procedure of ultraviolet-crosslinked
poly(A)–RNA–protein complexes that enables global RBP detection with high
specificity. We apply serIC to the nuclei of proliferating K562 cells to
obtain the first human nuclear RNA interactome. The domain composition of the
382 identified nuclear RBPs markedly differs from previous IC experiments,
including few factors without known RNA-binding domains that are in good
agreement with computationally predicted RNA binding. serIC extends the number
of DNA–RNA-binding proteins (DRBPs), and reveals a network of RBPs involved in
p53 signalling and double-strand break repair. serIC is an effective tool to
couple global RBP capture with additional selection or labelling steps for
specific detection of highly purified RBPs.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Biological sciences
dc.subject
Molecular biology
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Serial interactome capture of the human cell nucleus
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Nature Communications. - 7 (2015), Artikel Nr. 11212
dcterms.bibliographicCitation.doi
10.1038/ncomms11212
dcterms.bibliographicCitation.url
http://www.nature.com/ncomms/2016/160404/ncomms11212/full/ncomms11212.html
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000024547
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006415
dcterms.accessRights.openaire
open access