dc.contributor.author
Pawar, Kamlesh
dc.contributor.author
Hanisch, Carlos
dc.contributor.author
Palma Vera, Sergio Eliseo
dc.contributor.author
Einspanier, Ralf
dc.contributor.author
Sharbati, Soroush
dc.date.accessioned
2018-06-08T03:13:51Z
dc.date.available
2016-02-23
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14737
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18927
dc.description.abstract
Small non-coding RNA play a major part in host response to bacterial agents.
However, the role of long non-coding RNA (lncRNA) in this context remains
unknown. LncRNA regulate gene expression by acting e.g. as transcriptional
coactivators, RNA decoys or microRNA sponges. They control development,
differentiation and cellular processes such as autophagy in disease
conditions. Here, we provide an insight into the role of lncRNA in
mycobacterial infections. Human macrophages were infected with Mycobacterium
bovis BCG and lncRNA expression was studied early post infection. For this
purpose, lncRNA with known immune related functions were preselected and a
lncRNA specific RT-qPCR protocol was established. In addition to expression-
based prediction of lncRNA function, we assessed strategies for thorough
normalisation of lncRNA. Arrayed quantification showed infection-dependent
repression of several lncRNA including MEG3. Pathway analysis linked MEG3 to
mTOR and PI3K-AKT signalling pointing to regulation of autophagy. Accordingly,
IFN-γ induced autophagy in infected macrophages resulted in sustained MEG3
down regulation and lack of IFN-γ allowed for counter regulation of MEG3 by
viable M. bovis BCG. Knockdown of MEG3 in macrophages resulted in induction of
autophagy and enhanced eradication of intracellular M. bovis BCG.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Down regulated lncRNA MEG3 eliminates mycobacteria in macrophages via
autophagy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 6 (2016), Artikel Nr. 19416
dc.identifier.sepid
48056
dcterms.bibliographicCitation.doi
10.1038/srep19416
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1038/srep19416
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Veterinär-Biochemie
refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
refubium.funding.id
2100012210
refubium.mycore.fudocsId
FUDOCS_document_000000023796
refubium.note.author
Gefördert durch die DFG und den Open-Access-Publikationsfonds der Freien
Universität Berlin
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005904
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
2045-2322