dc.contributor.author
Winter, Oliver
dc.contributor.author
Musiol, Stephanie
dc.contributor.author
Schablowsky, Melissa
dc.contributor.author
Cheng, Qingyu
dc.contributor.author
Khodadadi, Laleh
dc.contributor.author
Hiepe, Falk
dc.date.accessioned
2018-06-08T03:13:30Z
dc.date.available
2015-11-06T07:26:26.492Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14713
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18903
dc.description.abstract
Introduction While protective plasma cells (PCs) are an important part of the
individual’s immune defense, autoreactive plasma cells such as dsDNA-specific
plasma cells contribute to the pathogenesis of autoimmune diseases like
systemic lupus erythematosus (SLE). However, the research on dsDNA-specific
plasma cells was restricted to the ELISpot technique, with its limitations, as
no other attempt for identification of dsDNA-reactive plasma cells had been
successful. Methods With improved fluorochrome labeling of dsDNA, removal of
DNA aggregates, and enhanced blocking of unspecific binding, we were able to
specifically detect dsDNA-reactive plasma cells by immunofluorescence
microscopy. Results Via this novel technique we were able to distinguish
short-lived (SLPCs) and long-lived (LLPCs) autoreactive plasma cells,
discriminate dsDNA-specific plasma cells according to their immunoglobulin
class (IgG, IgM, and IgA) and investigate autoreactive (dsDNA) and vaccine-
induced ovalbumin (Ova) plasma cells in parallel. Conclusions The detection of
autoreactive dsDNA-specific plasma cells via immunofluorescence microscopy
allows specific studies on pathogenic and protective plasma cell subsets and
their niches, detailed evaluation of therapeutic treatments and therefore
offers new possibilities for basic and clinical research.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Autoantibodies
dc.subject
Long-lived plasma cells
dc.subject
Immunofluorescence microscopy
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via
immunofluorescence microscopy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Arthritis Research & Therapy. - 17(2015), 1, Artikel Nr. 293
dcterms.bibliographicCitation.doi
10.1186/s13075-015-0811-2
dcterms.bibliographicCitation.url
http://arthritis-research.com/content/17/1/293
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000023425
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005637
dcterms.accessRights.openaire
open access