dc.contributor.author
Megges, Matthias
dc.contributor.author
Oreffo, Richard O. C.
dc.contributor.author
Adjaye, James
dc.date.accessioned
2018-06-08T03:13:08Z
dc.date.available
2016-06-24T11:14:11.038Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14702
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18892
dc.description.abstract
Human bone mesenchymal stromal cells derived from fetal femur 55 days post-
conception were reprogrammed to induced pluripotent stem cells using episomal
plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC
and supplemented with the following pathway inhibitors — TGFβ receptor
inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3β inhibitor (CHIR99021) and
ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell
lines was demonstrated in vitro and by the Pluritest.
en
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Episomal plasmid-based generation of induced pluripotent stem cells from fetal
femur-derived human mesenchymal stromal cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Stem Cell Research. - 16 (2016), 1, S. 128–132
dcterms.bibliographicCitation.doi
10.1016/j.scr.2015.12.013
dcterms.bibliographicCitation.url
http://www.sciencedirect.com/science/article/pii/S1873506115001932
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000024895
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006687
dcterms.accessRights.openaire
open access
