dc.contributor.author
Jagielski, Michal
dc.contributor.author
Wolf, Johannes
dc.contributor.author
Marzahn, Ulrike
dc.contributor.author
Völker, Anna
dc.contributor.author
Lemke, Marion
dc.contributor.author
Meier, Carola
dc.contributor.author
Ertel, Wolfgang
dc.contributor.author
Godkin, Owen
dc.contributor.author
Arens, Stephan
dc.contributor.author
Schulze-Tanzil, Gundula
dc.date.accessioned
2018-06-08T03:07:55Z
dc.date.available
2014-11-14T14:23:38.530Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14551
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18743
dc.description.abstract
Chondrogenic differentiated mesenchymal stromal cells (MSCs) are a promising
cell source for articular cartilage repair. This study was undertaken to
determine the effectiveness of two three-dimensional (3D) culture systems for
chondrogenic MSC differentiation in comparison to primary chondrocytes and to
assess the effect of Interleukin (IL)-10 and Tumor Necrosis Factor (TNF)α on
chondrogenesis by MSCs in 3D high-density (H-D) culture. MSCs were isolated
from femur spongiosa, characterized using a set of typical markers and
introduced in scaffold-free H-D cultures or non-woven polyglycolic acid (PGA)
scaffolds for chondrogenic differentiation. H-D cultures were stimulated with
recombinant IL-10, TNFα, TNFα + IL-10 or remained untreated. Gene and protein
expression of type II collagen, aggrecan, sox9 and TNFα were examined. MSCs
expressed typical cell surface markers and revealed multipotency. Chondrogenic
differentiated cells expressed cartilage-specific markers in both culture
systems but to a lower extent when compared with articular chondrocytes.
Chondrogenesis was more pronounced in PGA compared with H-D culture. IL-10
and/or TNFα did not impair the chondrogenic differentiation of MSCs. Moreover,
in most of the investigated samples, despite not reaching significance level,
IL-10 had a stimulatory effect on the type II collagen, aggrecan and TNFα
expression when compared with the respective controls.
en
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
The Influence of IL-10 and TNFα on Chondrogenesis of Human Mesenchymal Stromal
Cells in Three-Dimensional Cultures
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Molecular Sciences. - 15 (2014), 9, S. 15821-15844
dcterms.bibliographicCitation.doi
10.3390/ijms150915821
dcterms.bibliographicCitation.url
http://www.mdpi.com/1422-0067/15/9/15821
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000021297
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004152
dcterms.accessRights.openaire
open access