dc.contributor.author
Nourbakhsh, Mahnaz
dc.contributor.author
Jaafari, Mahmoud Reza
dc.contributor.author
Lage, Hermann
dc.contributor.author
Abnous, Khalil
dc.contributor.author
Mosaffa, Fatemeh
dc.contributor.author
Badiee, Ali
dc.contributor.author
Behravan, Javad
dc.date.accessioned
2018-06-08T03:03:28Z
dc.date.available
2015-06-26T08:10:40.987Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14404
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18598
dc.description.abstract
Objective(s): P-glycoprotein (P-gp) is an efflux protein, the overexpression
of which has been associated with multidrug resistance in various cancers.
Although siRNA delivery to reverse P-gp expression may be promising for
sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of siRNA
requires effective carriers that can deliver siRNA intracellularly with
minimal toxicity on target cells. We investigated a special class of PEGylated
lipid-based nanoparticles (NP), named nanolipoparticles (NLPs), for siRNA-
mediated P-gp downregulation. Materials and Methods: NLPs were prepared based
on low detergent dialysis method. After characterization, we evaluated the
effect of NLPs on siRNA delivery, and P-gp downregulation compared to
oligofectamineTM (OFA) in vitro and in vivo. Results: Our results showed a
significant decrease in P-gp expression and subsequent enhancement of
chemosensitivity to doxorubicin in vitro. Although the effectiveness of NLPs
for in vitro siRNA delivery compared to OFA was limited, the results of in
vivo studies showed noticeable effectiveness of NLPs for systemic siRNA
delivery. siRNA delivery using NLPs could downregulate MDR1 in tumor cells
more than 80%, while OFA had a reverse effect on MDR1 expression in vivo.
Conclusion: The results indicated that the prepared NLPs could be suitable
siRNA delivery systems for tumor therapy.
en
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/de/
dc.subject
Multidrug resistance
dc.subject
siRNA delivery
dc.subject
Tumor targeting
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Nanolipoparticles-mediated MDR1 siRNA delivery reduces doxorubicin resistance
in breast cancer cells and silences MDR1 expression in xenograft model of
human breast cancer
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Iranian Journal of Basic Medical Sciences. - 18 (2015), 4, S. 385-392
dcterms.bibliographicCitation.url
http://ijbms.mums.ac.ir/article_4288_595.html
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022706
refubium.note.author
Der Artikel wurde in einer Open-Access-Zetschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005107
dcterms.accessRights.openaire
open access