dc.contributor.author
Schubert, Carola
dc.contributor.author
Raparelli, Valeria
dc.contributor.author
Westphal, Christina
dc.contributor.author
Dworatzek, Elke
dc.contributor.author
Petrov, George
dc.contributor.author
Kararigas, Georgios
dc.contributor.author
Regitz-Zagrosek
dc.date.accessioned
2018-06-08T03:00:59Z
dc.date.available
2016-11-03T11:04:12.390Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14323
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18517
dc.description.abstract
Background Estrogen improves cardiac recovery after ischemia/reperfusion (I/R)
by yet incompletely understood mechanisms. Mitochondria play a crucial role in
I/R injury through cytochrome c-dependent apoptosis activation. We tested the
hypothesis that 17β-estradiol (E2) as well as a specific ERβ agonist improve
cardiac recovery through estrogen receptor (ER)β-mediated mechanisms by
reducing mitochondria-induced apoptosis and preserving mitochondrial
integrity. Methods We randomized ovariectomized C57BL/6N mice 24h before I/R
to pre-treatment with E2 or a specific ERβ agonist (ERβA). Isolated hearts
were perfused for 20min prior to 30min global ischemia followed by 40min
reperfusion. Results Compared with controls, ERβA and E2 treated groups showed
a significant improvement in cardiac recovery, i.e. an increase in left
ventricular developed pressure, dP/dtmax and dP/dtmin. ERβA and E2 pre-
treatment led to a significant reduction in apoptosis with decreased
cytochrome c release from the mitochondria and increased mitochondrial levels
of anti-apoptotic Bcl2 and ACAA2. Protein levels of mitochondrial translocase
inner membrane (TIM23) and mitochondrial complex I of respiratory chain were
increased by ERβA and E2 pre-treatment. Furthermore, we found a significant
increase of myosin light chain 2 (MLC2) phosphorylation together with ERK1/2
activation in E2, but not in ERβA treated groups. Conclusions Activation of
ERβ is essential for the improvement of cardiac recovery after I/R through the
inhibition of apoptosis and preservation of mitochondrial integrity and can be
a achieved by a specific ERβ agonist. Furthermore, E2 modulates MLC2
activation after I/R independent of ERβ.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Estrogen receptor β
dc.subject
Ischemia/reperfusion
dc.subject
Myosin light chain
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Reduction of apoptosis and preservation of mitochondrial integrity under
ischemia/reperfusion injury is mediated by estrogen receptor β
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Biology of Sex Differences. - 7 (2016), Artikel Nr. 53
dcterms.bibliographicCitation.doi
10.1186/s13293-016-0104-8
dcterms.bibliographicCitation.url
http://bsd.biomedcentral.com/articles/10.1186/s13293-016-0104-8
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025653
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007300
dcterms.accessRights.openaire
open access