dc.contributor.author
Kinkley, Sarah
dc.contributor.author
Helmuth, Johannes
dc.contributor.author
Polansky, Julia K.
dc.contributor.author
Dunkel, Ilona
dc.contributor.author
Gasparoni, Gilles
dc.contributor.author
Froehler, Sebastian
dc.contributor.author
Chen, Wei
dc.contributor.author
Walter, Joern
dc.contributor.author
Hamann, Alf
dc.contributor.author
Chung, Ho-Ryun
dc.date.accessioned
2018-06-08T02:57:07Z
dc.date.available
2016-09-19T09:28:59.481Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/14196
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-18393
dc.description.abstract
The combinatorial action of co-localizing chromatin modifications and
regulators determines chromatin structure and function. However, identifying
co-localizing chromatin features in a high-throughput manner remains a
technical challenge. Here we describe a novel reChIP-seq approach and tailored
bioinformatic analysis tool, normR that allows for the sequential enrichment
and detection of co-localizing DNA-associated proteins in an unbiased and
genome-wide manner. We illustrate the utility of the reChIP-seq method and
normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in
primary human CD4+ memory T cells. We unravel widespread bivalency at
hypomethylated CpG-islands coinciding with inactive promoters of developmental
regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the
population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-
seq tracks. Finally, we provide evidence that bivalency is established and
stabilized by an interplay between the genome and epigenome. Our reChIP-seq
approach augments conventional ChIP-seq and is broadly applicable to unravel
combinatorial modes of action.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4+ memory
T cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Nature Communications. - 7 (2016), Artikel Nr. 12514
dcterms.bibliographicCitation.doi
10.1038/ncomms1251
dcterms.bibliographicCitation.url
http://www.nature.com/ncomms/2016/160817/ncomms12514/full/ncomms12514.html
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025384
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006972
dcterms.accessRights.openaire
open access