dc.contributor.author
Schwenke, Jörg
dc.date.accessioned
2018-06-08T00:57:32Z
dc.date.available
2000-12-14T00:00:00.649Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/12743
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-16941
dc.description
Die komplette Dissertation im pdf-Format (1.307.069 Bytes):
schwe.pdf
dc.description.abstract
Minipig breeds are being used as experimental animals in medico-biological
research. The cardiovascular system of the pig frequently serves as the model
for the human cardiovascular system. Histopathological myocardial alterations
which may have an impact on the survivability in stress situations can be
proven in clinically healthy fattened pigs. The objective is to examine
whether similar myocardial alterations are present in minipigs. These
processes must be distinguished from phenomena which result in the course of
the use of the animals for research purposes. The present thesis is thus
aiming at the completion of the spectrum of criteria concerning the
experimental animal Berlin minipig.
The hearts of 87 Berlin minipigs were examined. They were classified into
different age groups (1, 2, 3, 6, 12, 24 months). 9 and/or 8 myocardium
samples taken from each heart were prepared with hematoxylin and eosin
staining, and 1 with Sudan red G staining. The following criteria were
considered in the histological examination: inflammations, degenerations and
necroses, fatty degenerations, dystrophic calcinoses, sarcosporidiosis as well
as vascular alterations. The findings were collected semiquantatively by
classifying them into different degrees of severity, they were compared with
each other and statistically evaluated. For 35 hearts, the Luxol Fast Blue
staining was applied in addition. The method of stunning was taken into
account when the evaluation was made.
The histological examination showed that pathological myocardial findings can
be detected in clinically healthy Berlin minipigs. These alterations are age-
dependent. Inflammatory processes had the highest incidence. The portion of
tissue samples showing the said pathohistological alterations was 3.4 % of the
tissue samples examined in the first month of life, it rose to a maximum of
38.9% at an age of 12 months and it fell to 24.4% in the 24th month of life.
The portion of tissue samples showing degenerative and necrotic alterations
was 25.6% at an age of 12 months, and 14.4% at an age of 24 months of life. No
degenerative necrotic alterations were found in the 1 month old animals.
Degeneration and necrosis were always connected with inflammatory alterations.
Dystrophic calcinosis was only found in one tissue sample of the 6-month-old
Berlin minipigs. No degenerative fatty infiltration was found in any of the
preparations. The portion of tissue samples with Sarcosporidia cysts varied
between 0% (1 and 2 months) and 30% (12 months). Muscular-elastic intima
proliferations at the intramural coronary arteries were found with a higher
incidence with increasing age. The degree of severity of these processes was
age-dependent, too. The first presence of swellings of the intima and of the
media was proven in 6-month-old animals (10.5% of the hearts). At an age of 12
and 24 months, their share was 50% each. Intima-media swellings were
exclusively found in the papillary muscles.
The LFB staining is used for the detection of the myofibrillar degeneration as
a form of early myocardial damage. The myofibrillar degeneration may occur in
a diffuse form or as a myofibrillar degeneration of the transverse band type.
An LFB-positive myocardial damage could be proven in 82.9% of the hearts that
were examined. The animals stunned by the captive bolt pistol showed 75% of
LFB-positive myocardial damage, and the animals stunned by electroshock had a
positive reaction to LFB of 93.3%. Two thirds (66.7%) of the pigs stunned by
means of electroshock showed a moderate reaction. 13.3% of the hearts showed a
moderate to severe myocardial damage. 6.7% each of the hearts of the pigs that
had been stunned by means of electroshock showed a mild and a mild to moderate
damage. In 6.7% of the hearts of this group no LFB-positive reaction could be
determined. 25% of the animals stunned by the captive bolt pistol showed a
moderate damage. 15% of them had the transitional form of a mild to moderate
damage. In 25% of the hearts of this examination group no LFB-positive damages
at all could be proven. In one preparation each of the two groups that were
examined, the myofibrillar degeneration of the transverse band type was proven
whereas the remaining LFB-positive myocardial samples showed the diffuse type.
An artifical damage of the cardiac muscle by the stunning and killing process
itself seems to be given. Consequently, the significance of LFB staining for
the determination of early myocardial damage in killed miniature pigs not
caused by stunning is to be termed unreliable.
The results show that pathohistological myocardial alterations can be proven
in clinically healthy Berlin minipigs. The incidence and the degree of
severity of the pathomorphological alterations are at the same level as was
also determined for slaughter pigs of a different genetic stock. Infectious,
infectious-toxic and metabolism-induced factors as well as repeated stress-
induced micronecroses must be taken into consideration as the cause of the
said alterations. When using the Berlin minipig in biomedical research, the
presence of these natural myocardial alterations must be taken into account.
en
dc.rights.uri
http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen
dc.subject
histopathology
dc.subject
miniature-pigs
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Pathohistologische Myokardveränderungen bei Berliner Miniaturschweinen (Mini-
Lewe) unter Berücksichtigung verschiedener Altersklassen
dc.contributor.firstReferee
Univ.-Prof. Dr. V. Bergmann
dc.contributor.furtherReferee
Univ.-Prof. Dr. R. Berg
dc.date.accepted
1998-11-27
dc.date.embargoEnd
2001-02-05
dc.identifier.urn
urn:nbn:de:kobv:188-1998000848
dc.title.translated
Pathohistological myocardial alterations in Berlin-minipigs (Mini-Lewe) with
regard to different age groups
en
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDISS_thesis_000000000039
refubium.mycore.transfer
http://www.diss.fu-berlin.de/1998/84/
refubium.mycore.derivateId
FUDISS_derivate_000000000039
dcterms.accessRights.dnb
free
dcterms.accessRights.openaire
open access