dc.contributor.author
Dietrich, Tamara
dc.contributor.author
Aigner, Annette
dc.contributor.author
Hildebrandt, Alexander
dc.contributor.author
Weber, Jérôme
dc.contributor.author
Meyer Günderoth, Mara
dc.contributor.author
Hohlbaum, Katharina
dc.contributor.author
Keller, Johannes
dc.contributor.author
Tsitsilonis, Serafeim
dc.contributor.author
Maleitzke, Tazio
dc.date.accessioned
2025-09-17T08:27:13Z
dc.date.available
2025-09-17T08:27:13Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/49349
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-49071
dc.description.abstract
Objective animal health evaluation is essential to determine welfare and discomfort in preclinical in vivo research. Body condition scores, body weight, and grimace scales are commonly used to evaluate well-being in murine rheumatoid arthritis (RA) and osteoarthritis experiments. However, nest-building, a natural behavior in mice, has not yet been evaluated in wild type (WT) or genetically modified rodents suffering from collagen antibody-induced arthritis (CAIA). To address this, we analyzed nesting behavior in WT mice, calcitonin gene-related peptide alpha-deficient (alpha CGRP-/-) mice, and calcitonin receptor-deficient (Calcr-/-) mice suffering from experimental RA compared to healthy control (CTRL) groups of the same genotypes. CAIA was induced in 10-12-week-old male mice, and clinical parameters (body weight, grip strength, clinical arthritis score, ankle size) as well as nesting behavior were assessed over 10 or 48 days. A slight positive association between the nest score and body weight and grip strength was found for animals suffering from CAIA. For the clinical arthritis score and ankle size, no significant associations were observed. Mixed model analyses confirmed these associations. This study demonstrates that clinical effects of RA, such as loss of body weight and grip strength, might negatively affect nesting behavior in mice. Assessing nesting behavior in mice with arthritis could be an additional, non-invasive and thus valuable health parameter in future experiments to monitor welfare and discomfort in mice. During severe disease stages, pre-formed nest-building material may be provided to animals suffering from arthritis.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Antibodies / pharmacology
en
dc.subject
Arthritis, Experimental
en
dc.subject
Nesting Behavior
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Nesting behavior is associated with body weight and grip strength loss in mice suffering from experimental arthritis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
23087
dcterms.bibliographicCitation.doi
10.1038/s41598-023-49720-y
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Springer Nature
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
Springer Nature DEAL
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
38155203
dcterms.isPartOf.eissn
2045-2322