dc.contributor.author
Werhahn Beining, Marco
dc.contributor.author
Guenther, Sebastian
dc.contributor.author
Lübke-Becker, Antina
dc.contributor.author
Heiden, Stefan E.
dc.contributor.author
Schaufler, Katharina
dc.contributor.author
Kreienbrock, Lothar
dc.contributor.author
Schwabe, Michael
dc.date.accessioned
2025-09-08T13:19:04Z
dc.date.available
2025-09-08T13:19:04Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/49169
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-48892
dc.description.abstract
Antimicrobial resistance (AMR) is a growing concern in veterinary and public health, with extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli playing a significant role. This study examined 1,000 healthy and sick dogs from a veterinary clinic in northern Germany and identified 85 ESBL-producing E. coli. Whole-genome sequencing of these isolates revealed seven phylogroups. A (38.8%) and B1 (32.9%) were the most common. Multilocus sequence typing identified 42 sequence types (STs), with the globally occurring lineages ST744 and ST10 being predominant. Single nucleotide polymorphism analysis showed the clonal circulation of ST744 among dogs in shared environments, such as households or breeders, whereas ST10 isolates displayed greater genetic diversity. ST131, a pathogenic international high-risk clonal lineage often associated with humans, was assigned to one isolate. Virulence-associated genes (VAGs) were abundant across the isolates, with siderophore systems, biofilm formation, and adherence traits being prominent. All isolates carried enterobactin genes with additional siderophore systems, such as yersiniabactin and aerobactin, present in 36 isolates. The highest number of VAGs (25) was observed in isolates belonging to the pathogenic lineages ST648 and ST405. Sixty-nine percent of the isolates were multidrug-resistant, carrying resistance genes for three or more antibiotic classes, with beta-lactam, aminoglycoside, and tetracycline resistance being the most frequent. This study highlights globally occurring E. coli lineages in companion animals and the role of close contact environments in their dissemination. Although dog-to-human transmission was not investigated in this study, these findings support the need for a One Health approach to address AMR, emphasizing the interconnected health of humans, animals, and the environment.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
antibiotic resistance
en
dc.subject
ESBL Escherichia coli
la
dc.subject
beta-lactamase
en
dc.subject
drug resistance
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Genotypic characterization of extended-spectrum beta-lactamase-producing E. coli from dogs in northern Germany
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1128/spectrum.00087-25
dcterms.bibliographicCitation.journaltitle
Microbiology Spectrum
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.1128/spectrum.00087-25
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2165-0497
refubium.resourceType.provider
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