dc.contributor.author
Fu, Yulin
dc.contributor.author
Deng, Zhaoju
dc.contributor.author
Shen, Yingbo
dc.contributor.author
Wei, Weizhou
dc.contributor.author
Xiang, Qiumei
dc.contributor.author
Liu, Zhiyang
dc.contributor.author
Hanf, Kunning
dc.contributor.author
Huang, Suli
dc.contributor.author
Lv, Zexun
dc.contributor.author
Cao, Tingting
dc.contributor.author
Peng, Changfeng
dc.contributor.author
Zhang, Rong
dc.contributor.author
Zou, Xuan
dc.contributor.author
Shen, Jianzhong
dc.contributor.author
Schwarz, Stefan
dc.contributor.author
Wang, Yang
dc.contributor.author
Liu, Dejun
dc.contributor.author
Lv, Ziquan
dc.contributor.author
Ke, Yuebin
dc.date.accessioned
2025-01-06T12:48:41Z
dc.date.available
2025-01-06T12:48:41Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/46119
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-45829
dc.description.abstract
Background: The emergence of optrA, which can confer resistance to phenicols and oxazolidinones in Enterococcus spp., poses a growing public health threat.
Methods: 102 optrA-positive enterococci (OPEs) including various species were isolated from feces of 719 healthy volunteers in a Shenzhen community, China. Antimicrobial susceptibility of these isolates was tested. Whole-genome sequencing and bioinformatics analysis were performed to characterize molecular epidemiology of OPEs.
Results: Compared to optrA-negative enterococci (ONEs), antimicrobial resistance (linezolid, florfenicol, doxycycline, erythromycin and ciprofloxacin) and presence of antimicrobial resistance genes (ARGs) (fexA, cat, tet(M), erm(A), erm(B) and etc) were higher in OPEs. Phylogenetic analysis revealed that high similarly (19–338 SNPs) was observed between the optrA-positive E. faecalis from community and the strains from patients, animals, and environment. In 102 OPEs, the optrA gene was detected on the chromosome (n = 36), on plasmids (n = 62), or both (n = 4). A diverse range of optrA-carrying plasmid types was identified. The rep9-plasmid replicons were widely detected in E. faecalis (44/66), whereas repUS1-plasmid replicons were widely identified in other enterococcal species (7/66). Most of all ARGs harbored by isolates were co-existed on optrA-carrying plasmids, suggesting that the acquisition of optrA-carrying plasmids will pose a greater threat to public health. Notably, the pAD1 (rep9 family) + DOp1-type plasmids should receive more attention for the transfer of optrA given their high prevalence (36.36%), high number of co-located ARGs with optrA (83.87% of total ARGs) and presence in multiple sources. Tn6674, IS1216E, ISEnfa1 and ISEnfa5 are related to the transfer of chromosomal and plasmids-derived optrA, respectively. The bcrABDR gene cluster, fexA, and erm(A) were frequently identified surrounding optrA and may be transferred with optrA via IS1216E or ISEnfa1.
Conclusion: The transfer of optrA gene is related to a variety of mobile elements (including plasmids, insertion sequences, transposons), which will promote the horizontal transfer of optrA. Moreover, many ARGs co-exist with optrA and could co-transfer with optrA. The acquisition of OPEs and optrA-carrying plasmids will pose a greater threat to public health and should be obtained more attention, especially optrA-positive E. faecalis and pAD1 + DOp1-type plasmids.
en
dc.format.extent
13 Seiten
dc.rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
oxazolidinone
en
dc.subject
antibiotic resistance
en
dc.subject
Enterococcus
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
High prevalence and plasmidome diversity of optrA-positive enterococci in a Shenzhen community, China
dc.type
Wissenschaftlicher Artikel
dc.date.updated
2025-01-03T08:05:45Z
dcterms.bibliographicCitation.articlenumber
1505107
dcterms.bibliographicCitation.doi
10.3389/fmicb.2024.1505107
dcterms.bibliographicCitation.journaltitle
Frontiers in Microbiology
dcterms.bibliographicCitation.volume
15
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fmicb.2024.1505107
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1664-302X
refubium.resourceType.provider
DeepGreen