Optimized platform for automated glycan assembly

Abstract

Fast, affordable, and reliable access to complex glycans is essential to meet the demand for custom-made research tools for glycosciences. Automated glycan assembly (AGA), based on the solid-phase synthesis paradigm allows quickly producing a range of biologically relevant oligosaccharides. The instrument sets the limits as to the types of chemistries that can be executed. The new, smaller, and more economical synthesis device allows quick temperature adjustments between −40°C and +90°C based on an energy-efficient local-temperature-controlled (LTC) reactor. The power of the new instrument is illustrated in the context of several AGA syntheses of complex oligosaccharides.