Development of sound production in Danionella cerebrum

Abstract

Acoustic signalling, integral to intraspecific communication and reproductive behaviour, undergoes notable changes during an animal's ontogenetic development. The onset and progression of this maturation in fish remains poorly understood. Here, we investigated the ontogeny of acoustic communication in the miniature teleost Danionella cerebrum, one of the smallest known vertebrates and an emerging model organism. Its adult males produce audible clicks that appear in sequences with a repetition rate of ∼60 or ∼120 Hz, caused by consecutive unilateral or alternating bilateral compressions of the swim bladder. To investigate the maturation of this ability, we performed long-term sound recordings and morphological studies of the sound production apparatus in D. cerebrum throughout its ontogenetic development. We found that fish start producing clicks during the second month of their lives and continually increase their abundance and structured repetition over the course of the following 1 to 2 months. The sound production machinery, including specialised bone and cartilage structures, starts to form in males after approximately 4 weeks and prior to reaching sexual maturity. Although clicks increase in amplitude as animals mature, click repetition rates of 60 and 120 Hz are stable throughout development. This suggests fully mature pattern generation in juvenile males, yet a continued development of the drumming apparatus capable of creating louder sounds.