dc.contributor.author
Mugo, Robert M.
dc.contributor.author
Rausch, Sebastian
dc.contributor.author
Musimbi, Zaneta D.
dc.contributor.author
Strube, Christina
dc.contributor.author
Raulf, Marie-Kristin
dc.contributor.author
Landt, Olfert
dc.contributor.author
Gichuki, Paul M.
dc.contributor.author
Ebner, Friederike
dc.contributor.author
Mwacharo, Jedidah
dc.contributor.author
Hartmann, Susanne
dc.date.accessioned
2024-09-11T07:35:56Z
dc.date.available
2024-09-11T07:35:56Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/44894
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-44604
dc.description.abstract
Background
The standard diagnosis of Ascaris lumbricoides and other soil-transmitted helminth (STH) infections relies on the detection of worm eggs by copromicroscopy. However, this method is dependent on worm patency and shows only limited accuracy in low-intensity infection settings. We aimed to decipher the diagnostic accuracy of different antibodies using various Ascaris antigens in reference to copromicroscopy and quantitative PCR (qPCR), four months after national STH preventative chemotherapy among school children in western Kenya.
Methodology
STH infection status of 390 school children was evaluated via copromicroscopy (Kato-Katz and mini-FLOTAC) and qPCR. In parallel, Ascaris-specific antibody profiles against larval and adult worm lysates, and adult worm excretory-secretory (ES) products were determined by enzyme-linked immunosorbent assay. Antibody cross-reactivity was evaluated using the closely related zoonotic roundworm species Toxocara cati and Toxocara canis. The diagnostic accuracy of each antibody was evaluated using receiver operating curve analysis and the correspondent area under the curve (AUC).
Principal findings
Ascaris was the predominant helminth infection with an overall prevalence of 14.9% (58/390). The sensitivity of mini-FLOTAC and Kato-Katz for Ascaris diagnosis reached only 53.5% and 63.8%, respectively compared to qPCR. Although being more sensitive, qPCR values correlated with microscopic egg counts (R = -0.71, P<0.001), in contrast to antibody levels. Strikingly, IgG antibodies recognizing the ES products of adult Ascaris worms reliably diagnosed active Ascaris infection as determined by qPCR and microscopy, with IgG1 displaying the highest accuracy (AUC = 0.83, 95% CI: 0.75–0.91).
Conclusion
IgG1 antibody responses against adult Ascaris-ES products hold a promising potential for complementing the standard fecal and molecular techniques employed for monitoring Ascaris infections. This is of particular importance in the context of deworming programs as the antibody diagnostic accuracy was independent of egg counts.
en
dc.format.extent
18 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Antibody response
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Evaluation of copromicroscopy, multiplex-qPCR and antibody serology for monitoring of human ascariasis in endemic settings
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0012279
dcterms.bibliographicCitation.doi
10.1371/journal.pntd.0012279
dcterms.bibliographicCitation.journaltitle
PLOS Neglected Tropical Diseases
dcterms.bibliographicCitation.number
6
dcterms.bibliographicCitation.volume
18
dcterms.bibliographicCitation.url
https://doi.org/10.1371/journal.pntd.0012279
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Immunologie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1935-2735
refubium.resourceType.provider
WoS-Alert