dc.contributor.author
Roigas, Sophie
dc.contributor.author
Kakularam, Kumar R.
dc.contributor.author
Rothe, Michael
dc.contributor.author
Heydeck, Dagmar
dc.contributor.author
Aparoy, Polamarasetty
dc.contributor.author
Kuhn, Hartmut
dc.date.accessioned
2024-07-26T10:31:55Z
dc.date.available
2024-07-26T10:31:55Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/44318
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-44030
dc.description.abstract
The human genome involves six functional arachidonic acid lipoxygenase (ALOX) genes and the corresponding enzymes (ALOX15, ALOX15B, ALOX12, ALOX12B, ALOXE3, ALOX5) have been implicated in cell differentiation and in the pathogenesis of inflammatory, hyperproliferative, metabolic, and neurological disorders. In other vertebrates, ALOX-isoforms have also been identified, but they occur less frequently. Since bony fish represent the most abundant subclass of vertebrates, we recently expressed and characterized putative ALOX15 orthologs of three different bony fish species (Nothobranchius furzeri, Pundamilia nyererei, Scleropages formosus). To explore whether these enzymes represent functional equivalents of mammalian ALOX15 orthologs, we here compared a number of structural and functional characteristics of these ALOX-isoforms with those of mammalian enzymes. We found that in contrast to mammalian ALOX15 orthologs, which exhibit a broad substrate specificity, a membrane oxygenase activity, and a special type of dual reaction specificity, the putative bony fish ALOX15 orthologs strongly prefer C-20 fatty acids, lack any membrane oxygenase activity and exhibit a different type of dual reaction specificity with arachidonic acid. Moreover, mutagenesis studies indicated that the Triad Concept, which explains the reaction specificity of all mammalian ALOX15 orthologs, is not applicable for the putative bony fish enzymes. The observed functional differences between putative bony fish ALOX15 orthologs and corresponding mammalian enzymes suggest a targeted optimization of the catalytic properties of ALOX15 orthologs during vertebrate development.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
lipoxygenase
en
dc.subject
enzyme evolution
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Bony Fish Arachidonic Acid 15-Lipoxygenases Exhibit Different Catalytic Properties than Their Mammalian Orthologs, Suggesting Functional Enzyme Evolution during Vertebrate Development
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
14154
dcterms.bibliographicCitation.doi
10.3390/ijms241814154
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
18
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
24
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
37762455
dcterms.isPartOf.eissn
1422-0067