dc.contributor.author
Oldemeyer, Sabine
dc.contributor.author
La Greca, Mariafrancesca
dc.contributor.author
Langner, Pit
dc.contributor.author
Lê Công, Karoline-Luisa
dc.contributor.author
Schlesinger, Ramona
dc.contributor.author
Heberle, Joachim
dc.date.accessioned
2024-07-29T12:17:17Z
dc.date.available
2024-07-29T12:17:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/44052
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43761
dc.description.abstract
The ability to track minute changes of a single amino acid residue in a cellular environment is causing a paradigm shift in the attempt to fully understand the responses of biomolecules that are highly sensitive to their environment. Detecting early protein dynamics in living cells is crucial to understanding their mechanisms, such as those of photosynthetic proteins. Here, we elucidate the light response of the microbial chloride pump NmHR from the marine bacterium Nonlabens marinus, located in the membrane of living Escherichia coli cells, using nanosecond time-resolved UV/vis and IR absorption spectroscopy over the time range from nanoseconds to seconds. Transient structural changes of the retinal cofactor and the surrounding apoprotein are recorded using light-induced time-resolved UV/vis and IR difference spectroscopy. Of particular note, we have resolved the kinetics of the transient deprotonation of a single cysteine residue during the photocycle of NmHR out of the manifold of molecular vibrations of the cells. These findings are of high general relevance, given the successful development of optogenetic tools from photoreceptors to interfere with enzymatic and neuronal pathways in living organisms using light pulses as a noninvasive trigger.
en
dc.format.extent
10 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Infrared light
en
dc.subject
Peptides and proteins
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Nanosecond Transient IR Spectroscopy of Halorhodopsin in Living Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1021/jacs.4c03891
dcterms.bibliographicCitation.journaltitle
Journal of the American Chemical Society
dcterms.bibliographicCitation.number
28
dcterms.bibliographicCitation.pagestart
19118
dcterms.bibliographicCitation.pageend
19127
dcterms.bibliographicCitation.volume
146
dcterms.bibliographicCitation.url
https://doi.org/10.1021/jacs.4c03891
refubium.affiliation
Physik
refubium.funding
ACS Publications
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1520-5126