dc.contributor.author
Becker, Christin
dc.contributor.author
Adam, Aysegül
dc.contributor.author
Dommisch, Henrik
dc.contributor.author
Stach, Thomas
dc.contributor.author
Schaefer, Arne S.
dc.date.accessioned
2024-04-26T09:18:45Z
dc.date.available
2024-04-26T09:18:45Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43368
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43084
dc.description.abstract
Background: Entamoeba gingivalis (E. gingivalis) is an anaerobic protozoan that is strongly associated with inflamed periodontal pockets. It is able to invade the mucosal epithelium of the human host, where it can feed on epithelial cells and elicit a severe innate immune response. Unlike other Entamoeba species, it is considered that E. gingivalis cannot form cysts, because it is a non-infectious protozoan. The lack of encystation capability would make it susceptible to periodontal treatment. However, it is not clear how the human host becomes infected with E. gingivalis trophozoites. We investigated the ability of E. gingivalis to encapsulate in response to an unfavorable environment in vitro.
Methods: Different strains of E. gingivalis, isolated from inflamed periodontal pocket samples, were cultured for 8 days in the presence or absence of the antimicrobials amoxycillin and metronidazole. To reveal cyst formation, we investigated the morphology and ultrastructure of the amoeba by light, fluorescence, transmission and scanning electron microscopy. We also used the fluorescent dye calcofluor white M2R to demonstrate chitin present in the cyst wall.
Results: We observed exocysts and an intra-cystic space separating the encapsulated trophozoite from the environment. Remarkably, cysts showed a smooth surface, polygonal edges and smaller size compared to free-living trophozoites. In addition, encapsulated trophozoites that detached from the cyst wall had a dense cytoplasma without phagocytic vesicles. The cyst walls consisted of chitin as in other Entamoba species. The encapsulated trophozoids were mononuclear after antibioticinduced encapsulation.
Discussion: We conclude that E. gingivalis cyst formation has significant implications for dissemination and infection and may explain why established treatment approaches often fail to halt periodontal tissue destruction during periodontitis and peri-implantitis.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Entamoeba gingivalis
en
dc.subject
periodontitis
en
dc.subject
periimplantitis
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
In vitro induction of Entamoeba gingivalis cyst-like structures from trophozoites in response to antibiotic treatment
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1201394
dcterms.bibliographicCitation.doi
10.3389/fcimb.2023.1201394
dcterms.bibliographicCitation.journaltitle
Frontiers in Cellular and Infection Microbiology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
37469604
dcterms.isPartOf.eissn
2235-2988