dc.contributor.author
Singto, Tichakorn
dc.contributor.author
Filor, Viviane
dc.contributor.author
Vidak, Jonathan
dc.contributor.author
Klopfleisch, Robert
dc.contributor.author
Bäumer, Wolfgang
dc.date.accessioned
2024-03-01T07:00:50Z
dc.date.available
2024-03-01T07:00:50Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42586
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-42310
dc.description.abstract
Background
Itch sensitization has been reported in patients with chronic allergic skin diseases and observed in a mouse model of allergic contact dermatitis (ACD). There is evidence suggesting that neuroimmune interactions may contribute to itch sensitization, as an increase in dendritic cells (DCs) within ganglia has been observed during allergic conditions. However, how DCs interact with sensory neurons in ganglia during allergic conditions is still not known. This study aims to investigate the role of DCs in dorsal root ganglion (DRG) under ACD conditions, specifically focusing on itch sensitization within the DRG. The tolylene-2,4-diisocyanate (TDI) mouse model for ACD and the co-culture model of DCs and DRG neurons was employed in this study.
Results
We successfully induced ACD by TDI, as evidenced by the development of edema, elevated total serum IgE levels, and an observed itch reaction in TDI-sensitized mice. Calcium imaging and RT-qPCR analysis revealed that TDI-sensitized mice exhibited signs of peripheral sensitization, including a higher percentage of neurons responding to pruritogens and increased activation and expression of itch receptors in excised DRG of TDI-sensitized mice. Immunofluorescence and flow cytometric analysis displayed an increase of MHCII+ cells, which serves as a marker for DCs, within DRG during ACD. The co-culture study revealed that when DRG neurons were cultured with DCs, there was an increase in the number of neurons responsive to pruritogens and activation of itch receptors such as TRPA1, TRPV1, H1R, and TRPV4. In addition, the immunofluorescence and RT-qPCR study confirmed an upregulation of TRPV4.
Conclusions
Our findings indicate that there is an increase of MHCII+ cells and itch peripheral sensitization in DRG under TDI-induced ACD condition. It has been found that MHCII+ cells in DRG might contribute to the itch peripheral sensitization by activating itch receptors, as shown through co-culture studies between DRG neurons and DCs. Further studies are required to identify the specific mediator(s) responsible for peripheral sensitization induced by activated DCs.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Dendritic cell
en
dc.subject
Dorsal root ganglia
en
dc.subject
Peripheral sensitization
en
dc.subject
Allergic contact dermatitis
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Dendritic cells under allergic condition enhance the activation of pruritogen-responsive neurons via inducing itch receptors in a co-culture study
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
17
dcterms.bibliographicCitation.doi
10.1186/s12865-024-00604-4
dcterms.bibliographicCitation.journaltitle
BMC Immunology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
25
dcterms.bibliographicCitation.url
https://doi.org/10.1186/s12865-024-00604-4
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Pharmakologie und Toxikologie
refubium.affiliation.other
Institut für Tierpathologie
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1471-2172