dc.contributor.author
Sire, Lucas
dc.contributor.author
Schmidt Yanez, Paul
dc.contributor.author
Bezier, Annie
dc.contributor.author
Courtial, Beatrice
dc.contributor.author
Mbedi, Susan
dc.contributor.author
Sparmann, Sarah
dc.contributor.author
Larrieu, Laurent
dc.contributor.author
Rougerie, Rodolphe
dc.contributor.author
Bouget, Christophe
dc.contributor.author
Monaghan, Michael T.
dc.date.accessioned
2024-02-08T07:13:25Z
dc.date.available
2024-02-08T07:13:25Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42366
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-42091
dc.description.abstract
Background
Broad-scale monitoring of arthropods is often carried out with passive traps (e.g., Malaise traps) that can collect thousands of specimens per sample. The identification of individual specimens requires time and taxonomic expertise, limiting the geographical and temporal scale of research and monitoring studies. DNA metabarcoding of bulk-sample homogenates has been found to be faster, efficient and reliable, but the destruction of samples prevents a posteriori validation of species occurrences and relative abundances. Non-destructive metabarcoding of DNA extracted from collection medium has been applied in a limited number of studies, but further tests of efficiency are required with different trap types and collection media to assess the consistency of the method.
Methods
We quantified the detection rate of arthropod species when applying non-destructive DNA metabarcoding with a short (127-bp) fragment of mitochondrial COI on two combinations of passive traps and collection media: (1) water with monopropylene glycol (H2O–MPG) used in window-flight traps (WFT, 53 in total); (2) ethanol with monopropylene glycol (EtOH–MPG) used in Malaise traps (MT, 27 in total). We then compared our results with those obtained for the same samples using morphological identification (for WFTs) or destructive metabarcoding of bulk homogenate (for MTs). This comparison was applied as part of a larger study of arthropod species richness in silver fir (Abies alba Mill., 1759) stands across a range of climate-induced tree dieback levels and forest management strategies.
Results
Of the 53 H2O-MPG samples from WFTs, 16 produced no metabarcoding results, while the remaining 37 samples yielded 77 arthropod MOTUs in total, of which none matched any of the 343 beetle species morphologically identified from the same traps. Metabarcoding of 26 EtOH–MPG samples from MTs detected more arthropod MOTUs (233) than destructive metabarcoding of homogenate (146 MOTUs, 8 orders), of which 71 were shared MOTUs, though MOTU richness per trap was similar between treatments. While we acknowledge the failure of metabarcoding from WFT-derived collection medium (H2O–MPG), the treatment of EtOH-based Malaise trapping medium remains promising. We conclude however that DNA metabarcoding from collection medium still requires further methodological developments and cannot replace homogenate metabarcoding as an approach for arthropod monitoring. It can be used nonetheless as a complementary treatment when enhancing the detection of soft-bodied arthropods like spiders and Diptera.
en
dc.format.extent
30 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Bulk metabarcoding
en
dc.subject
Malaise traps
en
dc.subject
Preservative ethanol
en
dc.subject
Window-flight traps
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e16022
dcterms.bibliographicCitation.doi
10.7717/peerj.16022
dcterms.bibliographicCitation.journaltitle
PeerJ
dcterms.bibliographicCitation.volume
11
dcterms.bibliographicCitation.url
https://doi.org/10.7717/peerj.16022
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2167-8359
refubium.resourceType.provider
WoS-Alert