We investigate the non-relativistic limit of the Klein–Gordon equation for mixed scalar particles and show that, in this regime, one unavoidably arrives at redefining the particle’s inertial mass. This happens because, in contrast to the case when mixing is absent, the antiparticle sector contribution cannot be neglected for particles with definite flavor. To clearly demonstrate this feature, we adopt the Feshbach–Villars formalism for Klein–Gordon particles. Furthermore, within the same framework, we also demonstrate that, in the presence of a weak gravitational field, the mass parameter that couples to gravity (gravitational mass) does not match the effective inertial mass. This, in turn, implies a violation of the weak equivalence principle. Finally, we prove that the Bargmann’s superselection rule, which prohibits oscillating particles on the basis of the Galilean transformation, is incompatible with the non-relativistic limit of the Lorentz transformation and hence does not collide with the results obtained.
