dc.contributor.author
Jaarsma, Ate H.
dc.contributor.author
Zervas, Athanasios
dc.contributor.author
Sipes, Katie
dc.contributor.author
Campuzano Jiménez, Francisco
dc.contributor.author
Smith, Andrea Claire
dc.contributor.author
Svendsen, Liessel Victoria
dc.contributor.author
Thøgersen, Mariane S.
dc.contributor.author
Stougaard, Peter
dc.contributor.author
Benning, Liane G.
dc.contributor.author
Tranter, Martyn
dc.date.accessioned
2024-02-06T14:42:49Z
dc.date.available
2024-02-06T14:42:49Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42331
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-42056
dc.description.abstract
The Greenland Ice Sheet is a biome which is mainly microbially driven. Several different niches can be found within the glacial biome for those microbes able to withstand the harsh conditions, e.g., low temperatures, low nutrient conditions, high UV radiation in summer, and contrasting long and dark winters. Eukaryotic algae can form blooms during the summer on the ice surface, interacting with communities of bacteria, fungi, and viruses. Cryoconite holes and snow are also habitats with their own microbial community. Nevertheless, the microbiome of supraglacial habitats remains poorly studied, leading to a lack of representative genomes from these environments. Under-investigated extremophiles, like those living on the Greenland Ice Sheet, may provide an untapped reservoir of chemical diversity that is yet to be discovered. In this study, an inventory of the biosynthetic potential of these organisms is made, through cataloging the presence of biosynthetic gene clusters in their genomes. There were 133 high-quality metagenome-assembled genomes (MAGs) and 28 whole genomes of bacteria obtained from samples of the ice sheet surface, cryoconite, biofilm, and snow using culturing-dependent and -independent approaches. AntiSMASH and BiG-SCAPE were used to mine these genomes and subsequently analyze the resulting predicted gene clusters. Extensive sets of predicted Biosynthetic Gene Clusters (BGCs) were collected from the genome collection, with limited overlap between isolates and MAGs. Additionally, little overlap was found in the biosynthetic potential among different environments, suggesting specialization of organisms in specific habitats. The median number of BGCs per genome was significantly higher for the isolates compared to the MAGs. The most talented producers were found among Proteobacteria. We found evidence for the capacity of these microbes to produce antimicrobials, carotenoid pigments, siderophores, and osmoprotectants, indicating potential survival mechanisms to cope with extreme conditions. The majority of identified BGCs, including those in the most prevalent gene cluster families, have unknown functions, presenting a substantial potential for bioprospecting. This study underscores the diverse biosynthetic potential in Greenland Ice Sheet genomes, revealing insights into survival strategies and highlighting the need for further exploration and characterization of these untapped resources.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
supraglacial habitats
en
dc.subject
extremophiles
en
dc.subject
metagenomic sequencing
en
dc.subject
biosynthetic gene clusters
en
dc.subject
genome mining
en
dc.subject
bioprospecting
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
The undiscovered biosynthetic potential of the Greenland Ice Sheet microbiome
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1285791
dcterms.bibliographicCitation.doi
10.3389/fmicb.2023.1285791
dcterms.bibliographicCitation.journaltitle
Frontiers in Microbiology
dcterms.bibliographicCitation.volume
14
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fmicb.2023.1285791
refubium.affiliation
Geowissenschaften
refubium.affiliation.other
Institut für Geologische Wissenschaften / Fachrichtung Geochemie, Hydrogeologie, Mineralogie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1664-302X
refubium.resourceType.provider
WoS-Alert