dc.contributor.author
Göhler, Friedemann
dc.contributor.author
Corman, Victor M.
dc.contributor.author
Bleicker, Tobias
dc.contributor.author
Stroux, Andrea
dc.contributor.author
Dewey, Marc
dc.contributor.author
Diekhoff, Torsten
dc.date.accessioned
2023-12-13T14:13:01Z
dc.date.available
2023-12-13T14:13:01Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/41866
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-41587
dc.description.abstract
Background: During the current severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic, computed tomography (CT) has become widely used in patients with suspected or known coronavirus disease 2019 (COVID-19). This prospective observational study in 28 invasively ventilated and 18 non-invasively ventilated patients with confirmed SARS-CoV-2 contamination aims at investigating SARS-CoV-2 contamination of CT scanner surfaces and its infectiousness.
Methods: Swab sampling of the CT table and gantry before and after CT examinations was performed. Additionally, the CT ventilation system air grid was wiped off after each examination. Real-time reverse-transcription polymerase chain reaction (RT-PCR) for SARS-CoV-2 RNA (ribonucleic acid) and viral cell culture were performed in the virology core lab.
Results: After examination of non-invasively ventilated or non-ventilated patients, SARS-CoV-2 RNA was found in 11.1% (4/36) on patient near surfaces (CT table and gantry) and in 16.7% (3/18) on the CT air grid respectively after examination of invasively ventilated patients in 5.4% (3/56) on CT table and gantry and 7.1% (2/28) on the CT air grid. Surface contamination was more common in non-invasively ventilated or non-ventilated patients with a high viral load who were actively coughing. RT-PCR cycle threshold (Ct) was high (35.96-39.31) in all positive samples and no positive viral cell culture was found.
Conclusion: Our study suggests that CT scanner surface contamination with SARS-CoV-2 is considerable and more common after examination of non-invasively ventilated or non-ventilated patients compared to invasively ventilated patients. However, no viral cell culture positivity was found, hence the infectious potential seems low.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Disease transmission (Infectious)
en
dc.subject
Cross infection
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Contamination of CT scanner surfaces with SARS-CoV-2 and infective potential after examination of invasively ventilated, non-invasively ventilated and non-ventilated patients with positive throat swabs: prospective investigation using real-time reverse-transcription PCR and viral cell culture
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
61
dcterms.bibliographicCitation.doi
10.1186/s13244-022-01202-x
dcterms.bibliographicCitation.journaltitle
Insights into Imaging
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Springer Nature
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
Springer Nature DEAL
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
35347510
dcterms.isPartOf.eissn
1869-4101