dc.contributor.author
Neumann, Jonas
dc.contributor.author
Schmidtsdorff, Sebastian
dc.contributor.author
Schmidt, Alexander H.
dc.contributor.author
Parr, Maria K.
dc.date.accessioned
2023-11-24T08:34:12Z
dc.date.available
2023-11-24T08:34:12Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/41088
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-40809
dc.description.abstract
The purity analysis of therapeutic peptides can often be challenging, demanding the application of more than a single analytical technique. Supercritical fluid chromatography nowadays is a promising alternative to reversed-phase liquid chromatography, providing orthogonal and complementary information. This study investigated its applicability for the separation of human insulin, its analogs and degradation products. A previously published method development protocol for peptides up to 2000 Da was successfully applied to the higher molecular weight insulins (6 kDa). A single gradient method was optimized for all insulins using a Torus DEA column (100 × 3.0 mm, 1.7 μm), carbon dioxide and a modifier consisting of methanol/acetonitrile/water/methanesulfonic acid (65:35:2:0.1, v/v/v/v). Consecutively, the crown ether 18-crown-6, which is well known to complex charged lysine sidechains and other amino functionalities, was added to the modifier to evaluate its impact on selectivity. A decreased retention and a shift in the elution order for the insulins were observed. An inverse effect on retention was found when combined with a neutral stationary phase chemistry (Viridis BEH).
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
design of experiments
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Controlling the elution order of insulin and its analogs in sub-/supercritical fluid chromatography using methanesulfonic acid and 18-crown-6 as mobile phase additives
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2300520
dcterms.bibliographicCitation.doi
10.1002/jssc.202300520
dcterms.bibliographicCitation.journaltitle
Journal of Separation Science
dcterms.bibliographicCitation.number
22
dcterms.bibliographicCitation.volume
46
dcterms.bibliographicCitation.url
https://doi.org/10.1002/jssc.202300520
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Pharmazie
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refubium.funding
DEAL Wiley
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1615-9314