Title:
Linker, loading, and reaction scale influence automated glycan assembly
Author(s):
Dal Colle, Marlene C. S.; Ricardo, Manuel G.; Hribernik, Nives; Danglad-Flores, José; Seeberger, Peter H.; Delbianco, Martina
Year of publication:
2023
Available Date:
2023-08-09T07:46:53Z
Abstract:
Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not significantly influence the AGA outcome, the chemical nature of the linker dramatically altered the isolated yields. We identified that the major determinants of AGA yields are cleavage from the solid support and post-AGA purification steps.
Part of Identifier:
e-ISSN (online): 1860-5397
Keywords:
automated glycan assembly
photocleavable linker
polysaccharides
solid-phase synthesis
DDC-Classification:
540 Chemie und zugeordnete Wissenschaften
Publication Type:
Wissenschaftlicher Artikel
URL of the Original Publication:
DOI of the Original Publication:
Journaltitle:
Beilstein Journal of Organic Chemistry
Department/institution:
Biologie, Chemie, Pharmazie
Institut für Chemie und Biochemie