dc.contributor.author
Thane, Michael
dc.contributor.author
Paisios, Emmanouil
dc.contributor.author
Stöter, Torsten
dc.contributor.author
Krüger, Anna-Rosa
dc.contributor.author
Gläß, Sebastian
dc.contributor.author
Dahse, Anne-Kristin
dc.contributor.author
Scholz, Nicole
dc.contributor.author
Gerber, Bertram
dc.contributor.author
Lehmann, Dirk J.
dc.contributor.author
Schleyer, Michael
dc.date.accessioned
2023-06-08T08:18:24Z
dc.date.available
2023-06-08T08:18:24Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39766
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39484
dc.description.abstract
Neuronally orchestrated muscular movement and locomotion are defining faculties of multicellular animals. Due to its simple brain and genetic accessibility, the larva of the fruit fly Drosophila melanogaster allows one to study these processes at tractable levels of complexity. However, although the faculty of locomotion clearly pertains to the individual, most studies of locomotion in larvae use measurements aggregated across animals, or animals tested one by one, an extravagance for larger-scale analyses. This prevents grasping the inter- and intra-individual variability in locomotion and its neurogenetic determinants. Here, we present the IMBA (individual maggot behaviour analyser) for analysing the behaviour of individual larvae within groups, reliably resolving individual identity across collisions. We use the IMBA to systematically describe the inter- and intra-individual variability in locomotion of wild-type animals, and how the variability is reduced by associative learning. We then report a novel locomotion phenotype of an adhesion GPCR mutant. We further investigated the modulation of locomotion across repeated activations of dopamine neurons in individual animals, and the transient backward locomotion induced by brief optogenetic activation of the brain-descending ‘mooncrawler’ neurons. In summary, the IMBA is an easy-to-use toolbox allowing an unprecedentedly rich view of the behaviour and its variability of individual larvae, with utility in multiple biomedical research contexts.
en
dc.format.extent
33 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
High-resolution analysis of individual Drosophila melanogaster larvae uncovers individual variability in locomotion and its neurogenetic modulation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
220308
dcterms.bibliographicCitation.doi
10.1098/rsob.220308
dcterms.bibliographicCitation.journaltitle
Open Biology
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.1098/rsob.220308
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2046-2441
refubium.resourceType.provider
WoS-Alert