dc.contributor.author
Müller, Paul Markus
dc.contributor.author
Rocks, Oliver
dc.date.accessioned
2023-04-27T15:19:40Z
dc.date.available
2023-04-27T15:19:40Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39152
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-38869
dc.description.abstract
Rho family GTPases are central regulators of cytoskeletal dynamics controlled by guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs). This protocol presents a workflow for a robust high-throughput compatible biosensor assay to analyze changes in Rho GTPase activity by these proteins in the native cellular environment. The procedure can be used for semi-quantitative comparison of GEF/GAP function and extended for analysis of additional modulators. The experimental design is applicable also to other monomolecular ratiometric FRET sensors. For complete details on the use and execution of this protocol, please refer to Müller et al. (2020).
en
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Environmental sciences
en
dc.subject
Material sciences
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
A screening-compatible live cell fluorescence resonance energy transfer-based assay for modulation of Rho GTPase activity
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
101705
dcterms.bibliographicCitation.doi
10.1016/j.xpro.2022.101705
dcterms.bibliographicCitation.journaltitle
STAR Protocols
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
Elsevier
dcterms.bibliographicCitation.volume
3
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
36149795
dcterms.isPartOf.issn
2666-1667