dc.contributor.author
Kohler, Joel
dc.contributor.author
Mei, Jie
dc.contributor.author
Banneke, Stefanie
dc.contributor.author
Winter, York
dc.contributor.author
Endres, Matthias
dc.contributor.author
Emmrich, Julius Valentin
dc.date.accessioned
2023-04-13T13:00:36Z
dc.date.available
2023-04-13T13:00:36Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38874
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-38590
dc.description.abstract
The radial arm maze (RAM) is a common behavioral test to quantify spatial learning and memory in rodents. Prior attempts to refine the standard experimental setup have been insufficient. Previously, we demonstrated the feasibility of a fully automated, voluntary, and stress-free eight-arm RAM not requiring food or water deprivation. Here, we compared this newly developed refined RAM to a classic manual experimental setup using 24 female 10-12 weeks old C57BL/6J mice. We used a lipopolysaccharide (LPS)-induced model of systemic inflammation to examine long-term cognitive impairment for up to 13 weeks following LPS injection. Both mazes demonstrated robust spatial learning performance during the working memory paradigm. The refined RAM detected spatial learning and memory deficits among LPS-treated mice in the working memory paradigm, whereas the classic RAM detected spatial learning and memory deficits only in the combined working/reference memory paradigm. In addition, the refined RAM allowed for quantification of an animal's overall exploratory behavior and day/night activity pattern. While our study highlights important aspects of refinement of the new setup, our comparison of methods suggests that both RAMs have their respective merits depending on experimental requirements.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
spatial learning
en
dc.subject
behavioral test
en
dc.subject
radial arm maze (RAM)
en
dc.subject
LPS (lipopolysaccharide)
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Assessing spatial learning and memory in mice: Classic radial maze versus a new animal-friendly automated radial maze allowing free access and not requiring food deprivation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1013624
dcterms.bibliographicCitation.doi
10.3389/fnbeh.2022.1013624
dcterms.bibliographicCitation.journaltitle
Frontiers in Behavioral Neuroscience
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
16
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
36248032
dcterms.isPartOf.eissn
1662-5153