dc.contributor.author
Zhang, Hui
dc.contributor.author
Beilfuss, Nirina
dc.contributor.author
Zabarylo, Urszula
dc.contributor.author
Raum, Kay
dc.contributor.author
Puts, Regina
dc.date.accessioned
2023-03-28T12:03:58Z
dc.date.available
2023-03-28T12:03:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38621
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-38337
dc.description.abstract
Quickly developing precision medicine and patient-oriented treatment strategies urgently require novel technological solutions. The randomly cell-populated scaffolds usually used for tissue engineering often fail to mimic the highly anisotropic characteristics of native tissue. In this work, an ultrasound standing-wave-based tissue engineering acoustophoretic (TEA) set-up was developed to organize murine mesenchymal stromal cells (mMSCs) in an in situ polymerizing 3-D fibrin hydrogel. The resultant constructs, consisting of 17 cell layers spaced at 300 mu m, were obtained by continuous wave ultrasound applied at a 2.5 MHz frequency. The patterned mMSCs preserved the structured behavior within 10 days of culturing in osteogenic conditions. Cell viability was moderately increased 1 day after the patterning; it subdued and evened out, with the cells randomly encapsulated in hydrogels, within 21 days of culturing. Cells in the structured hydrogels exhibited enhanced expression of certain osteogenic markers, i.e., Runt-related transcription factor 2 (RUNX2), osterix (Osx) transcription factor, collagen-1 alpha1 (COL1A1), osteopontin (OPN), osteocalcin (OCN), and osteonectin (ON), as well as of certain cell-cycle-progression-associated genes, i.e., Cyclin D1, cysteine-rich angiogenic inducer 61 (CYR61), and anillin (ANLN), when cultured with osteogenic supplements and, for ANLN, also in the expansion media. Additionally, OPN expression was also augmented on day 5 in the patterned gels cultured without the osteoinductive media, suggesting the pro-osteogenic influence of the patterned cell organization. The TEA set-up proposes a novel method for non-invasively organizing cells in a 3-D environment, potentially enhancing the regenerative properties of the designed anisotropic constructs for bone healing.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
acoustophoresis
en
dc.subject
tissue engineering
en
dc.subject
patterned 3-D construct
en
dc.subject
mesenchymal stromal cells
en
dc.subject
osteogenic differentiation
en
dc.subject
bone regeneration
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
A Tissue Engineering Acoustophoretic (TEA) Set-up for the Enhanced Osteogenic Differentiation of Murine Mesenchymal Stromal Cells (mMSCs)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
11473
dcterms.bibliographicCitation.doi
10.3390/ijms231911473
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
19
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
23
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
36232775
dcterms.isPartOf.eissn
1422-0067