dc.contributor.author
Poch, Franz G. M.
dc.contributor.author
Neizert, Christina Ann
dc.contributor.author
Geyer, B.
dc.contributor.author
Gemeinhardt, Ole
dc.contributor.author
Niehues, Stefan Markus
dc.contributor.author
Vahldiek, Janis Lucas
dc.contributor.author
Bressem, Keno Kyrill
dc.contributor.author
Lehmann, K. S.
dc.date.accessioned
2023-03-03T14:58:43Z
dc.date.available
2023-03-03T14:58:43Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38210
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37927
dc.description.abstract
Multibipolar radiofrequency ablation (RFA) is an advanced ablation technique for early stage hepatocellular carcinoma and liver metastases. Vessel cooling in multibipolar RFA has not been systematically investigated. The objective of this study was to evaluate the presence of perivascular vital cells within the ablation zone after multibipolar RFA. Multibipolar RFA were performed in domestic pigs in vivo. Three internally cooled bipolar RFA applicators were used simultaneously. Three experimental settings were planned: (1) inter-applicator-distance: 15 mm; (2) inter-applicator-distance: 20 mm; (3) inter-applicator-distance: 20 mm with hepatic inflow occlusion (Pringle maneuver). A vitality staining was used to analyze liver cell vitality around all vessels in the ablation center with a diameter>0.5 mm histologically. 771 vessels were identified. No vital tissue was seen around 423 out of 429 vessels (98.6%) situated within the central white zone. Vital cells could be observed around major hepatic vessels situated adjacent to the ablation center. Vessel diameter (>3.0 mm; p<0.05) and low vessel-to-ablation-center distance (<0.2 mm; p<0.05) were identified as risk factors for incomplete ablation adjacent to hepatic vessels. The vast majority of vessels, which were localized in the clinically relevant white zone, showed no vital perivascular cells, regardless of vessel diameter and vessel type. However, there was a risk of incomplete ablation around major hepatic vessels situated directly within the ablation center. A Pringle maneuver could avoid incomplete ablations.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Multibipolar radiofrequency ablation (RFA)
en
dc.subject
in vivo porcine model
en
dc.subject
Perivascular vital cells
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Perivascular vital cells in the ablation center after multibipolar radiofrequency ablation in an in vivo porcine model
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
13886
dcterms.bibliographicCitation.doi
10.1038/s41598-021-93406-2
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.originalpublishername
Springer Nature
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
Springer Nature DEAL
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
34230573
dcterms.isPartOf.eissn
2045-2322