dc.contributor.author
Möbius, Klaus
dc.contributor.author
Savitsky, Anton
dc.date.accessioned
2023-03-01T12:06:51Z
dc.date.available
2023-03-01T12:06:51Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37890
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37606
dc.description.abstract
During the last decades, the combined efforts of biologists, chemists, and physicists in developing high-field/high-frequency EPR techniques and applying them to functional proteins have demonstrated that this type of magnetic resonance spectroscopy is particularly powerful for characterizing the structure and dynamics of stable and transient states of proteins in action on biologically relevant time scales ranging from nanoseconds to hours. The review article describes how high-field EPR methodology, in conjunction with site-specific isotope and spin-labeling strategies, is capable of providing new insights into fundamental biological processes. Specifically, we discuss the theoretical and instrumental background of continuous-wave and pulse high-field EPR and the multiple-resonance extensions EDNMR, ENDOR, TRIPLE, ESEEM, PELDOR, and RIDME. Some emphasis is placed on a balanced description of both the historical spadework and the achieved performance of advanced EPR at 95 GHz and 360 GHz. This culminates in a coherent treatment of state-of-the-art research of high-field EPR in terms of both instrumentation development and application to representative protein complexes such as cofactor binding sites in photosynthesis.
en
dc.format.extent
81 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
High-field/High-frequency EPR Spectroscopy
en
dc.subject
Protein Research
en
dc.subject
Principles and Examples
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
High-field/High-frequency EPR Spectroscopy in Protein Research: Principles and Examples
dc.type
Wissenschaftlicher Artikel
dc.identifier.sepid
97136
dcterms.bibliographicCitation.doi
10.1007/s00723-022-01511-w
dcterms.bibliographicCitation.journaltitle
Applied Magnetic Resonance
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.pagestart
207
dcterms.bibliographicCitation.pageend
287
dcterms.bibliographicCitation.volume
54
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00723-022-01511-w
refubium.affiliation
Physik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1613-7507
refubium.resourceType.provider
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