dc.contributor.author
Obara, Isaiah
dc.contributor.author
Nijhof, Ard
dc.contributor.author
Atimnedi, Patrick
dc.contributor.author
Mijele, Domnic
dc.contributor.author
Nanteza, Anne
dc.contributor.author
Elati, Khawla
dc.contributor.author
Bishop, Richard
dc.date.accessioned
2023-04-12T05:38:45Z
dc.date.available
2023-04-12T05:38:45Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37805
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37518
dc.description.abstract
African buffalo (Syncerus caffer) have been distinct from the Auroch lineage leading to domestic cattle for 5 million years, and are reservoirs of multiple pathogens, that affect introduced domestic cattle. To date, there has been no analysis of the class I MHC locus in African buffalo. We present the first data on African buffalo class I MHC, which demonstrates that gene and predicted protein coding sequences are approximately 86–87% similar to that of African domestic cattle in the peptide binding region. The study also shows concordance in the distribution of codons with elevated posterior probabilities of positive selection in the buffalo class I MHC and known antigen binding sites in cattle. Overall, the diversity in buffalo class I sequences appears greater than that in cattle, perhaps related to a more complex pathogen challenge environment in Africa. However, application of NetMHCpan suggested broad clustering of peptide binding specificities between buffalo and cattle. Furthermore, in the case of at least 20 alleles, critical peptide-binding residues appear to be conserved with those of cattle, including at secondary anchor residues. Alleles with six different length transmembrane regions were detected. This preliminary analysis suggests that like cattle, but unlike most other mammals, African buffalo appears to exhibit configuration (haplotype) variation in which the loci are expressed in distinct combinations.
en
dc.format.extent
18 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
African buffalo (Syncerus caffer)
en
dc.subject
Theileria parva
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
The antigen recognition portion of African buffalo class I MHC is highly polymorphic, consistent with a complex pathogen challenge environment, and the 3’ region suggests distinct haplotype configurations
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s00251-022-01287-0
dcterms.bibliographicCitation.journaltitle
Immunogenetics
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.pagestart
115
dcterms.bibliographicCitation.pageend
132
dcterms.bibliographicCitation.volume
75
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00251-022-01287-0
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1432-1211