Title:
Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
Author(s):
Trumpp, Michael; Oliveras, Anna; Gonschior, Hannes; Ast, Julia; Hodson, David J.; Knaus, Petra; Lehmann, Martin; Birol, Melissa; Broichhagen, Johannes
Year of publication:
2022
Available Date:
2023-01-17T13:17:14Z
Abstract:
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.
Part of Identifier:
e-ISSN (online): 1364-548X
Keywords:
Förster Resonance Energy Transfer
near-infrared ATTO700
acceptor
DDC-Classification:
540 Chemie und zugeordnete Wissenschaften
Publication Type:
Wissenschaftlicher Artikel
URL of the Original Publication:
DOI of the Original Publication:
Journaltitle:
Chemical Communications
Department/institution:
Biologie, Chemie, Pharmazie
Institut für Chemie und Biochemie
