dc.contributor.author
Manz, Christian
dc.contributor.author
Götze, Michael
dc.contributor.author
Frank, Clemens
dc.contributor.author
Zappe, Andreas
dc.contributor.author
Pagel, Kevin
dc.date.accessioned
2022-07-04T09:38:58Z
dc.date.available
2022-07-04T09:38:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/35257
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34975
dc.description.abstract
LC–MS is one of the most important tools for the comprehensive characterization of N-glycans. Despite many efforts to speed up glycan analysis via optimized sample preparation (e.g., faster enzyme digestion in combination with instant or rapid labeling dyes), a major bottleneck remains the rather long measurement times of HILIC chromatography. Further complication arises from the necessity to concomitantly calibrate with an external standard to allow for accurate retention times and the conversion into more robust GU values. Here we demonstrate the use of an internal calibration strategy for HILIC chromatography to speed up glycan analysis. By reducing the number of utilized dextran oligosaccharides, the calibrant can be spiked directly into the sample such that external calibration runs are no longer required. The minimized dextran ladder shows accurate GU calibration with a minor deviation of well below 1% and can be applied without modifications in sample preparation or data processing. We further demonstrate the simultaneous use of the minimized dextran ladder as calibrant for the estimation of CCS values in traveling wave ion mobility spectrometry. In both cases, the minimized dextran ladder enables the measurement of calibrant and sample in a single HPLC run without losing information or accuracy.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
N-Glycan analysis
en
dc.subject
Ion mobility spectrometry
en
dc.subject
Glucose units
en
dc.subject
Collision cross sections
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Dextran as internal calibrant for N-glycan analysis by liquid chromatography coupled to ion mobility-mass spectrometry
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s00216-022-04133-0
dcterms.bibliographicCitation.journaltitle
Analytical and Bioanalytical Chemistry
dcterms.bibliographicCitation.number
17
dcterms.bibliographicCitation.pagestart
5023
dcterms.bibliographicCitation.pageend
5031
dcterms.bibliographicCitation.volume
414
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00216-022-04133-0
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1618-2650