dc.contributor.author
Kröger, Marius
dc.contributor.author
Scheffel, Jörg
dc.contributor.author
Nikolaev, Viktor V.
dc.contributor.author
Shirshin, Evgeny A.
dc.contributor.author
Siebenhaar, Frank
dc.contributor.author
Schleusener, Johannes
dc.contributor.author
Lademann, Jürgen
dc.contributor.author
Maurer, Marcus
dc.contributor.author
Darvin, Maxim E.
dc.date.accessioned
2022-05-18T11:56:39Z
dc.date.available
2022-05-18T11:56:39Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/35043
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34759
dc.description.abstract
Mast cells (MCs) are multifunctional cells of the immune system and are found in skin and all major tissues of the body. They contribute to the pathology of several diseases including urticaria, psoriasis, atopic dermatitis and mastocytosis where they are increased at lesional sites. Histomorphometric analysis of skin biopsies serves as a routine method for the assessment of MC numbers and their activation status, which comes with major limitations. As of now, non-invasive techniques to study MCs in vivo are not available. Here, we describe a label-free imaging technique to visualize MCs and their activation status in the human papillary dermis in vivo. This technique uses two-photon excited fluorescence lifetime imaging (TPE-FLIM) signatures, which are different for MCs and other dermal components. TPE-FLIM allows for the visualization and quantification of dermal MCs in healthy subjects and patients with skin diseases. Moreover, TPE-FLIM can differentiate between two MC populations in the papillary dermis in vivo—resting and activated MCs with a sensitivity of 0.81 and 0.87 and a specificity of 0.85 and 0.84, respectively. Results obtained on healthy volunteers and allergy and mastocytosis patients indicate the existence of other MC subpopulations within known resting and activated MC populations. The developed method may become an important tool for non-invasive in vivo diagnostics and therapy control in dermatology and immunology, which will help to better understand pathomechanisms involving MC accumulation, activation and degranulation and to characterize the effects of therapies that target MCs.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Case-Control Studies
en
dc.subject
Dermatitis, Atopic
en
dc.subject
Fluorescence
en
dc.subject
Hypersensitivity
en
dc.subject
Mastocytosis
en
dc.subject
Microscopy, Fluorescence, Multiphoton
en
dc.subject
Optical Imaging
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
14930
dcterms.bibliographicCitation.doi
10.1038/s41598-020-71901-2
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.originalpublishername
Springer Nature
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
Springer Nature DEAL
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32913196
dcterms.isPartOf.eissn
2045-2322