dc.contributor.author
Maibier, Martin
dc.contributor.author
Bintig, Willem
dc.contributor.author
Goede, Andrean
dc.contributor.author
Höpfner, Michael
dc.contributor.author
Kuebler, Wolfgang M.
dc.contributor.author
Secomb, Timothy W.
dc.contributor.author
Nitzsche, Bianca
dc.contributor.author
Pries, Axel R.
dc.date.accessioned
2022-03-03T12:32:41Z
dc.date.available
2022-03-03T12:32:41Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/34315
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34032
dc.description.abstract
Objective:
In this study, we examined the impact of gap junction blockade on chick chorioallantoic membrane microvessels.
Methods:
Expression of Cx37, Cx40/42, and Cx43 in chick chorioallantoic membrane tissue was studied by PCR, Western blot, and confocal immunofluorescence microscopy. Vessel diameter changes occurring under gap junction blockade with carbenoxolone (175 µmol/L), palmitoleic acid (100 µmol/L), 43GAP27 (1 mmol/L) were analyzed by intravital microscopy. To analyze vascular tone, chick chorioallantoic membrane vessels were exposed to a vasodilator cocktail consisting of acetylcholine (10 μmol/L), adenosine (100 μmol/L), papaverine (200 μmol/L), and sodium nitroprusside (10 μmol/L).
Results:
In chick chorioallantoic membrane lysates, Western blot analysis revealed the expression of Cx40 and Cx43. Immunofluorescence in intact chick chorioallantoic membrane vasculature showed only Cx43, limited to arterial vessel walls. Upon gap junction blockade (3 hours) arterial and venous diameters decreased to 0.50 ± 0.03 and 0.36 ± 0.06 (carbenoxolone), 0.72 ± 0.08 and 0.63 ± 0.15 (palmitoleic acid) and 0.77 ± 0.004 and 0.58 ± 0.05 (GAP27), relative to initial values. Initially, diameter decrease was dominated by increasing vascular tone. After 6 hours, however, vessel tone was reduced, suggesting structural network remodeling.
Conclusions:
Our findings suggest a major role for connexins in mediating acute and chronic diameter changes in developing vascular networks.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
carbenoxolone
en
dc.subject
chick chorioallantoic membrane
en
dc.subject
gap junctions
en
dc.subject
vascular remodeling
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Gap junctions regulate vessel diameter in chick chorioallantoic membrane vasculature by both tone‐dependent and structural mechanisms
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e12590
dcterms.bibliographicCitation.doi
10.1111/micc.12590
dcterms.bibliographicCitation.journaltitle
Microcirculation
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Wiley
dcterms.bibliographicCitation.volume
27
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31520425
dcterms.isPartOf.issn
1073-9688
dcterms.isPartOf.eissn
1549-8719