dc.contributor.author
Schinke, Christian
dc.contributor.author
Fernandez Vallone, Valeria
dc.contributor.author
Ivanov, Andranik
dc.contributor.author
Peng, Yangfan
dc.contributor.author
Körtvelyessy, Péter
dc.contributor.author
Nolte, Luca
dc.contributor.author
Huehnchen, Petra
dc.contributor.author
Beule, Dieter
dc.contributor.author
Stachelscheid, Harald
dc.contributor.author
Boehmerle, Wolfgang
dc.contributor.author
Endres, Matthias
dc.date.accessioned
2022-01-20T12:17:59Z
dc.date.available
2022-01-20T12:17:59Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/33655
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33375
dc.description.abstract
Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and potentially irreversible adverse event of cytotoxic chemotherapy. We evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for chemotherapy induced neurotoxicity. Sensory neurons differentiated from two established induced pluripotent stem cell lines were used (s.c. BIHi005-A https://hpscreg.eu/cell- line/BIHi005-A and BIHi004-B https://hpscreg.eu/cell-line/BIHi004-B, Berlin Institute of Health Stem Cell Core Facility). Cell viability and cytotoxicity assays were performed, comparing susceptibility to four neurotoxic and two non-neurotoxic drugs. RNA sequencing analyses in paclitaxel vs. vehicle (DMSO)treated sensory neurons were performed. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to a dose dependent decline of cell viability in clinically relevant IC50 ranges, which was not the case for the non-neurotoxic compounds doxorubicin and 5-fluorouracil. RNA sequencing analyses at 24 h, i.e. before paclitaxel-induced cell death occurred, revealed the differential expression of genes of neuronal injury, cellular stress response, and sterol pathways in response to 1 mu M paclitaxel. Neuroprotective effects of lithium chloride co-incubation, which were previously shown in rodent dorsal root ganglia, could be replicated in human iPSC-DSN. Cell lines from the two different donors BIHi005-A and BIHi004-B showed different responses to the neurotoxic treatment in cell viability and cytotoxicity assays.
en
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Induced pluripotent stem cell derived sensory neurons (iPSC-DSN)
en
dc.subject
Chemotherapy induced neuropathy
en
dc.subject
Transcriptome
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Dataset for: Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
107320
dcterms.bibliographicCitation.doi
10.1016/j.dib.2021.107320
dcterms.bibliographicCitation.journaltitle
Data in Brief
dcterms.bibliographicCitation.originalpublishername
Elsevier
dcterms.bibliographicCitation.volume
38
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
34485650
dcterms.isPartOf.eissn
2352-3409