dc.contributor.author
Kanevche, Katerina
dc.contributor.author
Burr, David
dc.contributor.author
Nürnberg, Dennis
dc.contributor.author
Haß, Pascal-Kolja
dc.contributor.author
Elsaesser, Andreas
dc.contributor.author
Heberle, Joachim
dc.date.accessioned
2022-01-05T12:10:17Z
dc.date.available
2022-01-05T12:10:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/33334
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33055
dc.description.abstract
Although techniques such as fluorescence-based super-resolution imaging or confocal microscopy simultaneously gather both morphological and chemical data, these techniques often rely on the use of localized and chemically specific markers. To eliminate this flaw, we have developed a method of examining cellular cross sections using the imaging power of scattering-type scanning near-field optical microscopy and Fourier-transform infrared spectroscopy at a spatial resolution far beyond the diffraction limit. Herewith, nanoscale surface and volumetric chemical imaging is performed using the intrinsic contrast generated by the characteristic absorption of mid-infrared radiation by the covalent bonds. We employ infrared nanoscopy to study the subcellular structures of eukaryotic (Chlamydomonas reinhardtii) and prokaryotic (Escherichia coli) species, revealing chemically distinct regions within each cell such as the microtubular structure of the flagellum. Serial 100 nm-thick cellular cross-sections were compiled into a tomogram yielding a three-dimensional infrared image of subcellular structure distribution at 20 nm resolution. The presented methodology is able to image biological samples complementing current fluorescence nanoscopy but at less interference due to the low energy of infrared radiation and the absence of labeling.
en
dc.format.extent
8 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
3-D reconstruction
en
dc.subject
Nanoscale biophysics
en
dc.subject
infrared nanoscopy
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Infrared nanoscopy and tomography of intracellular structures
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1341
dcterms.bibliographicCitation.doi
10.1038/s42003-021-02876-7
dcterms.bibliographicCitation.journaltitle
Communications Biology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
4
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s42003-021-02876-7
refubium.affiliation
Physik
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Anatomie
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
refubium.resourceType.provider
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