dc.contributor.author
Fulde, Marcus
dc.contributor.author
van Vorst, Kira
dc.contributor.author
Zhang, Kaiyi
dc.contributor.author
Westermann, Alexander J.
dc.contributor.author
Busche, Tobias
dc.contributor.author
Huei, Yong Chiun
dc.contributor.author
Welitschanski, Katharina
dc.contributor.author
Pägelow, Dennis
dc.contributor.author
Plendl, Johanna
dc.contributor.author
Tedin, Karsten
dc.date.accessioned
2021-11-25T13:46:32Z
dc.date.available
2021-11-25T13:46:32Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32859
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-32585
dc.description.abstract
Salmonella pathogenicity island (SPI) 2 type three secretion system (T3SS)-mediated effector molecules facilitate bacterial survival in phagocytes but their role in the intestinal epithelium in vivo remains ill-defined. Using our neonatal murine infection model in combination with SPI2 reporter technology and RNA-Seq of sorted primary enterocytes, we demonstrate expression of SPI2 effector molecules by intraepithelial Salmonella Typhimurium (S. Typhimurium). Contrary to expectation, immunostaining revealed that infection with SPI2 T3SS-mutants resulted in significantly enlarged intraepithelial Salmonella-containing vacuoles (SCV) with altered cellular positioning, suggesting impaired apical to basolateral transmigration. Also, infection with isogenic tagged S. Typhimurium strains revealed a reduced spread of intraepithelial SPI2 T3SS mutant S. Typhimurium to systemic body sites. These results suggest that SPI2 T3SS effector molecules contribute to enterocyte apical to basolateral transmigration of the SCV during the early stage of the infection.
en
dc.format.extent
15 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Salmonella pathogenicity island 2 (Spi-2)
en
dc.subject
apical to basolateral transmigration
en
dc.subject
mucosal translocation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1973836
dcterms.bibliographicCitation.doi
10.1080/19490976.2021.1973836
dcterms.bibliographicCitation.journaltitle
Gut Microbes
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.1080/19490976.2021.1973836
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen
refubium.affiliation.other
Institut für Veterinär-Anatomie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1949-0984
refubium.resourceType.provider
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