dc.contributor.author
Gollasch, Benjamin
dc.contributor.author
Wu, Guanlin
dc.contributor.author
Liu, Tong
dc.contributor.author
Dogan, Inci
dc.contributor.author
Rothe, Michael
dc.contributor.author
Gollasch, Maik
dc.contributor.author
Luft, Friedrich C.
dc.date.accessioned
2021-11-22T13:04:34Z
dc.date.available
2021-11-22T13:04:34Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32796
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-32522
dc.description.abstract
Fatty acid products derived from cytochromes P450 (CYP) monooxygenase and lipoxygenase (LOX)/CYP ω/(ω-1)-hydroxylase pathways are a superclass of lipid mediators with potent bioactivities. Whether or not the chronic kidney disease (CKD) and hemodialysis treatments performed on end-stage renal disease (ESRD) patients affect RBC epoxy fatty acids profiles remains unknown. Measuring the products solely in plasma is suboptimal. Since such determinations invariably ignore red blood cells (RBCs) that make up 3 kg of the circulating blood. RBCs are potential reservoirs for epoxy fatty acids that regulate cardiovascular function. We studied 15 healthy persons and 15 ESRD patients undergoing regular hemodialysis treatments. We measured epoxides derived from CYP monooxygenase and metabolites derived from LOX/CYP ω/(ω-1)-hydroxylase pathways in RBCs by LC–MS/MS tandem mass spectrometry. Our data demonstrate that various CYP epoxides and LOX/CYP ω/(ω-1)-hydroxylase products are increased in RBCs of ESRD patients, compared to control subjects, including dihydroxyeicosatrienoic acids (DHETs), epoxyeicosatetraenoic acids (EEQs), dihydroxydocosapentaenoic acids (DiHDPAs), and hydroxyeicosatetraenoic acids (HETEs). Hemodialysis treatment did not affect the majority of those metabolites. Nevertheless, we detected more pronounced changes in free metabolite levels in RBCs after dialysis, as compared with the total RBC compartment. These findings indicate that free RBC eicosanoids should be considered more dynamic or vulnerable in CKD.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
chronic kidney disease (CKD)
en
dc.subject
erythrocytes
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Hemodialysis and erythrocyte epoxy fatty acids
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e14601
dcterms.bibliographicCitation.doi
10.14814/phy2.14601
dcterms.bibliographicCitation.journaltitle
Physiological Reports
dcterms.bibliographicCitation.number
20
dcterms.bibliographicCitation.originalpublishername
Wiley
dcterms.bibliographicCitation.volume
8
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33112511
dcterms.isPartOf.eissn
2051-817X