dc.contributor.author
Ahlfeld, Tilman
dc.contributor.author
Lode, Anja
dc.contributor.author
Richter, Richard Frank
dc.contributor.author
Pradel, Winnie
dc.contributor.author
Franke, Adrian
dc.contributor.author
Rauner, Martina
dc.contributor.author
Stadlinger, Bernd
dc.contributor.author
Lauer, Günter
dc.contributor.author
Gelinsky, Michael
dc.contributor.author
Korn, Paula
dc.date.accessioned
2021-09-24T08:38:28Z
dc.date.available
2021-09-24T08:38:28Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32042
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31771
dc.description.abstract
Cleft alveolar bone defects can be treated potentially with tissue engineered bone grafts. Herein, we developed novel biphasic bone constructs consisting of two clinically certified materials, a calcium phosphate cement (CPC) and a fibrin gel that were biofabricated using 3D plotting. The fibrin gel was loaded with mesenchymal stromal cells (MSC) derived from bone marrow. Firstly, the degradation of fibrin as well as the behavior of cells in the biphasic system were evaluated in vitro. Fibrin degraded quickly in presence of MSC. Our results showed that the plotted CPC structure acted slightly stabilizing for the fibrin gel. However, with passing time and fibrin degradation, MSC migrated to the CPC surface. Thus, the fibrin gel could be identified as cell delivery system. A pilot study in vivo was conducted in artificial craniofacial defects in Lewis rats. Ongoing bone formation could be evidenced over 12 weeks but the biphasic constructs were not completely osseous integrated. Nevertheless, our results show that the combination of 3D plotted CPC constructs and fibrin as suitable cell delivery system enables the fabrication of novel regenerative implants for the treatment of alveolar bone defects.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cleft alveolar osteoplasty
en
dc.subject
bone tissue engineering
en
dc.subject
calcium phosphate cement
en
dc.subject
mesenchymal stem cells
en
dc.subject
biofabrication
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Toward Biofabrication of Resorbable Implants Consisting of a Calcium Phosphate Cement and Fibrin—A Characterization In Vitro and In Vivo
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1218
dcterms.bibliographicCitation.doi
10.3390/ijms22031218
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
3
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
22
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33530649
dcterms.isPartOf.eissn
1422-0067