dc.contributor.author
Knorr, Sarah
dc.contributor.author
Reissert-Oppermann, Sophia
dc.contributor.author
Tomás-Cortázar, Julen
dc.contributor.author
Barriales, Diego
dc.contributor.author
Azkargorta, Mikel
dc.contributor.author
Iloro, Ibon
dc.contributor.author
Elortza, Félix
dc.contributor.author
Pinecki-Socias, Sophia
dc.contributor.author
Anguita, Juan
dc.contributor.author
Hovius, Joppe W.
dc.contributor.author
Nijhof, Ard M.
dc.date.accessioned
2021-11-11T14:06:08Z
dc.date.available
2021-11-11T14:06:08Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/31436
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31169
dc.description.abstract
Ixodes ricinus is the main vector of tick-borne diseases in Europe. An immunization trial of calves with soluble extracts of I. ricinus salivary glands (SGE) or midgut (ME) previously showed a strong response against subsequent tick challenge, resulting in diminished tick feeding success. Immune sera from these trials were used for the co-immunoprecipitation of tick tissue extracts, followed by LC-MS/MS analyses. This resulted in the identification of 46 immunodominant proteins that were differentially recognized by the serum of immunized calves. Some of these proteins had previously also drawn attention as potential anti-tick vaccine candidates using other approaches. Selected proteins were studied in more detail by measuring their relative expression in tick tissues and RNA interference (RNAi) studies. The strongest RNAi phenotypes were observed for MG6 (A0A147BXB7), a protein containing eight fibronectin type III domains predominantly expressed in tick midgut and ovaries of feeding females, and SG2 (A0A0K8RKT7), a glutathione-S-transferase that was found to be upregulated in all investigated tissues upon feeding. The results demonstrated that co-immunoprecipitation of tick proteins with host immune sera followed by protein identification using LC-MS/MS is a valid approach to identify antigen–antibody interactions, and could be integrated into anti-tick vaccine discovery pipelines.
en
dc.format.extent
17 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Ixodes ricinus
en
dc.subject
immunoprecipitation
en
dc.subject
anti-tick vaccines
en
dc.subject
salivary glands
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Identification and Characterization of Immunodominant Proteins from Tick Tissue Extracts Inducing a Protective Immune Response against Ixodes ricinus in Cattle
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
636
dcterms.bibliographicCitation.doi
10.3390/vaccines9060636
dcterms.bibliographicCitation.journaltitle
Vaccines
dcterms.bibliographicCitation.number
6
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3390/vaccines9060636
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2076-393X