dc.contributor.author
Schuchardt, Mirjam
dc.contributor.author
Siegel, Nathalie Vanessa
dc.contributor.author
Babic, Milen
dc.contributor.author
Reshetnik, Alexander
dc.contributor.author
Lützenberg, Ronald
dc.contributor.author
Zidek, Walter
dc.contributor.author
Giet, Markus van der
dc.contributor.author
Tölle, Markus
dc.date.accessioned
2021-06-14T14:53:03Z
dc.date.available
2021-06-14T14:53:03Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/31024
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30760
dc.description.abstract
The investigation of vascular calcification and its underlying cellular and molecular pathways is of great interest in current research efforts. Therefore, suitable assays are needed to allow examination of the complex calcification process under controlled conditions. The current study describes a new ex vivo model of isolated-perfused rat aortic tissue with subsequent quantification and vessel staining to analyze the calcium content of the aortic wall. A rat aorta was perfused ex vivo with control and calcification media for 14 days, respectively. The calcification medium was luminally perfused and induced a significant increase in calcium deposition within the media of the vessel wall detected alongside the elastic laminae. Perfusion with control medium induced no calcification. In addition, the mRNA expression of the osteogenic marker bone morphogenetic protein 2 (BMP-2) increased in aortic tissue after perfusion, while SM22α as smooth muscle marker decreased. This newly developed ex vivo model of isolated-perfused rat aorta is suitable for vascular calcification studies testing inducers and inhibitors of vessel calcification and studying signaling pathways within calcification progression.
en
dc.subject
Aortic perfusion
en
dc.subject
Ex vivo mineralization
en
dc.subject
Smooth muscle cell
en
dc.subject
Vascular calcification
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
A Novel Long-Term ex vivo Model for Studying Vascular Calcification Pathogenesis: The Rat Isolated-Perfused Aorta
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1159/000503785
dcterms.bibliographicCitation.journaltitle
Journal of Vascular Research
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Karger
dcterms.bibliographicCitation.pagestart
46
dcterms.bibliographicCitation.pageend
52
dcterms.bibliographicCitation.volume
57
dcterms.rightsHolder.note
Copyright applies in this work.
dcterms.rightsHolder.url
http://rightsstatements.org/vocab/InC/1.0/
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.note.author
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
de
refubium.note.author
This publication is shared with permission of the rights owner and made freely accessible through a DFG (German Research Foundation) funded license at either an alliance or national level.
en
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31722349
dcterms.isPartOf.issn
1018-1172
dcterms.isPartOf.eissn
1423-0135