dc.contributor.author
Pöppe, Judith
dc.contributor.author
Bote, Katrin
dc.contributor.author
Ramesh, Abhinaya
dc.contributor.author
Murugaiyan, Jayaseelan
dc.contributor.author
Kuropka, Benno
dc.contributor.author
Kühl, Michael
dc.contributor.author
Johnston, Paul
dc.contributor.author
Roesler, Uwe
dc.contributor.author
Makarova, Olga
dc.date.accessioned
2021-04-27T10:44:06Z
dc.date.available
2021-04-27T10:44:06Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30556
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30296
dc.description.abstract
Evolution of bacterial tolerance to antimicrobials precedes evolution of resistance and may result in cross-tolerance, cross-resistance, or collateral sensitivity to other antibiotics. Transient exposure of gut bacteria to glyphosate, the world’s most widely used herbicide, has been linked to the activation of the stress response and changes in susceptibility to antibiotics. In this study, we investigated whether chronic exposure to a glyphosate-based herbicide (GBH) results in resistance, a constitutive activation of the tolerance and stress responses, and cross-tolerance or cross-resistance to antibiotics. Of the 10 farm animal-derived clinical isolates of Salmonella enterica subjected to experimental evolution in increasing concentrations of GBH, three isolates showed stable resistance with mutations associated with the glyphosate target gene aroA and no fitness costs. Global quantitative proteomics analysis demonstrated activation of the cellular tolerance and stress response during the transient exposure to GBH but not constitutively in the resistant mutants. Resistant mutants displayed no cross-resistance or cross-tolerance to antibiotics. These results suggest that while transient exposure to GBH triggers cellular tolerance response in Salmonella enterica, this response does not become genetically fixed after selection for resistance to GBH and does not result in increased cross-tolerance or cross-resistance to clinically important antibiotics under our experimental conditions.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Enterobacteriaceae
en
dc.subject
fitness costs
en
dc.subject
experimental evolution
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Selection for Resistance to a Glyphosate-Containing Herbicide in Salmonella enterica Does Not Result in a Sustained Activation of the Tolerance Response or Increased Cross-Tolerance and Cross-Resistance to Clinically Important Antibiotics
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e01204
dcterms.bibliographicCitation.doi
10.1128/AEM.01204-20
dcterms.bibliographicCitation.journaltitle
Applied and Environmental Microbiology
dcterms.bibliographicCitation.number
24
dcterms.bibliographicCitation.volume
86
dcterms.bibliographicCitation.url
https://doi.org/10.1128/AEM.01204-20
refubium.affiliation
Veterinärmedizin
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
refubium.affiliation.other
Institut für Biologie
refubium.funding
Publikationsfonds FU
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1098-5336