dc.contributor.author
Hahn, Marc Benjamin
dc.contributor.author
Dietrich, Paul M.
dc.contributor.author
Radnik, Jörg
dc.date.accessioned
2021-04-13T12:17:55Z
dc.date.available
2021-04-13T12:17:55Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30313
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30053
dc.description.abstract
Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS xperiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cancer therapy
en
dc.subject
Xray photo electron spectrocopy
en
dc.subject
hydration dependence
en
dc.subject
Single-strand break
en
dc.subject
Double-strand break
en
dc.subject
Hydrated DNA
en
dc.subject
Hydrated electron
en
dc.subject
Hydroxyl radical
en
dc.subject
Dissociative electron attachment
en
dc.subject
Prehydrated electron
en
dc.subject.ddc
500 Natural sciences and mathematics::540 Chemistry and allied sciences::541 Physical and theoretical chemistry
dc.title
In situ monitoring of the influence of water on DNA radiation damage by near-ambient pressure X-ray photoelectron spectroscopy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
50
dcterms.bibliographicCitation.doi
10.1038/s42004-021-00487-1
dcterms.bibliographicCitation.journaltitle
Communications Chemistry
dcterms.bibliographicCitation.volume
4
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s42004-021-00487-1
refubium.affiliation
Physik
refubium.affiliation.other
Institut für Experimentalphysik
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refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
refubium.funding.id
442240902/HA 8528/2-1
refubium.note.author
Open Access funding enabled and organized by Projekt DEAL.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2399-3669
dc.relation.isreviewedby
https://refubium.fu-berlin.de/handle/fub188/33085