dc.contributor.author
Tatarkova, Zuzana
dc.contributor.author
de Baaij, Jeroen H. F.
dc.contributor.author
Grendar, Marian
dc.contributor.author
Aschenbach, Jörg R.
dc.contributor.author
Racay, Peter
dc.contributor.author
Bos, Caro
dc.contributor.author
Sponder, Gerhard
dc.contributor.author
Hoenderop, Joost G. J.
dc.contributor.author
Röntgen, Monika
dc.contributor.author
Turcanova Koprusakova, Monika
dc.contributor.author
Kolisek, Martin
dc.date.accessioned
2021-02-12T14:02:39Z
dc.date.available
2021-02-12T14:02:39Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29617
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29361
dc.description.abstract
Cardiomyocytes are among the most energy-intensive cell types. Interplay between the components of cellular magnesium (Mg) homeostasis and energy metabolism in cardiomyocytes is poorly understood. We have investigated the effects of dietary Mg content and presence/functionality of the Na+/Mg2+ exchanger SLC41A1 on enzymatic functions of selected constituents of the Krebs cycle and complexes of the electron transport chain (ETC). The activities of aconitate hydratase (ACON), isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (KGDH), and ETC complexes CI–CV have been determined in vitro in mitochondria isolated from hearts of wild-type (WT) and Slc41a1−/− mice fed a diet with either normal or low Mg content. Our data demonstrate that both, the type of Mg diet and the Slc41a1 genotype largely impact on the activities of enzymes of the Krebs cycle and ETC. Moreover, a compensatory effect of Slc41a1−/− genotype on the effect of low Mg diet on activities of the tested Krebs cycle enzymes has been identified. A machine-learning analysis identified activities of ICDH, CI, CIV, and CV as common predictors of the type of Mg diet and of CII as suitable predictor of Slc41a1 genotype. Thus, our data delineate the effect of dietary Mg content and of SLC41A1 functionality on the energy-production in cardiac mitochondria.
en
dc.format.extent
25 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cardiomyocyte
en
dc.subject
electron transport chain
en
dc.subject
oxidative phosphorylation
en
dc.subject
Na+/Mg2+ exchanger
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Dietary Mg2+ Intake and the Na+/Mg2+ Exchanger SLC41A1 Influence Components of Mitochondrial Energetics in Murine Cardiomyocytes
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
8221
dcterms.bibliographicCitation.doi
10.3390/ijms21218221
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
21
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
21
dcterms.bibliographicCitation.url
https://doi.org/10.3390/ijms21218221
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Physiologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1422-0067