dc.contributor.author
Conradie, Andelé M.
dc.contributor.author
Bertzbach, Luca D.
dc.contributor.author
Trimpert, Jakob
dc.contributor.author
Patria, Joseph N.
dc.contributor.author
Murata, Shiro
dc.contributor.author
Parcells, Mark S.
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2021-02-10T07:32:59Z
dc.date.available
2021-02-10T07:32:59Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29569
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29313
dc.description.abstract
Modified-live herpesvirus vaccines are widely used in humans and animals, but field strains can emerge that have a higher virulence and break vaccinal protection. Since the introduction of the first vaccine in the 1970s, Marek's disease virus overcame the vaccine barrier by the acquisition of numerous genomic mutations. However, the evolutionary adaptations in the herpesvirus genome responsible for the vaccine breaks have remained elusive. Here, we demonstrate that point mutations in the multifunctional meq gene acquired during evolution can significantly alter virulence. Defined mutations found in highly virulent strains also allowed the virus to overcome innate cellular responses and vaccinal protection. Concomitantly, the adaptations in meq enhanced virus shedding into the environment, likely providing a selective advantage for the virus. Our study provides the first experimental evidence that few point mutations in a single herpesviral gene result in drastically increased virulence, enhanced shedding, and escape from vaccinal protection.
en
dc.format.extent
20 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Marek's disease virus
en
dc.subject
transformation
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e1009104
dcterms.bibliographicCitation.doi
10.1371/journal.ppat.1009104
dcterms.bibliographicCitation.journaltitle
PLOS Pathogens
dcterms.bibliographicCitation.number
12
dcterms.bibliographicCitation.volume
16
dcterms.bibliographicCitation.url
https://doi.org/10.1371/journal.ppat.1009104
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1553-7366
dcterms.isPartOf.eissn
1553-7374
refubium.resourceType.provider
WoS-Alert