Title:
Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research
Author(s):
Urner, Leonhard H.; Goltsche, Katharina; Selent, Marleen; Liko, Idlir; Schweder, Marc-Philip; Robinson, Carol V.; Pagel, Kevin; Haag, Rainer
Year of publication:
2021
Available Date:
2021-02-03T08:51:04Z
Abstract:
Dendrons are an important class of macromolecules that can be used for a broad range of applications. Recent studies have indicated that mixtures of oligoglycerol detergent (OGD) regioisomers are superior to individual regioisomers for protein extraction. The origin of this phenomenon remains puzzling. Here we discuss the synthesis and characterization of dendritic oligoglycerol regioisomer mixtures and their implementation into detergents. We provide experimental benchmarks to support quality control after synthesis and investigate the unusual utility of OGD regioisomer mixtures for extracting large protein quantities from biological membranes. We anticipate that our findings will enable the development of mixed detergent platforms in the future.
Part of Identifier:
e-ISSN (online): 1521-3765
Keywords:
dendrons
detergents
membrane protein purification
oligoglycerol
regioisomers
DDC-Classification:
540 Chemie und zugeordnete Wissenschaften
Publication Type:
Wissenschaftlicher Artikel
URL of the Original Publication:
DOI of the Original Publication:
Journaltitle:
Chemistry—A European Journal
Department/institution:
Biologie, Chemie, Pharmazie
Institut für Chemie und Biochemie
Comments:
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.